Тип публикации: статья из журнала
Год издания: 2023
Идентификатор DOI: 10.3390/ijms241914919
Аннотация: <jats:p>The synthesis of PHA was first investigated using WFOs obtained from smoked-sprat heads, substandard fresh sprats, and fresh mackerel heads and backbones. All the WFOs ensured the growth of the wild-type strain Cupriavidus necator B-10646 and the synthesis of PHA, regardless of the degree of lipid saturation (from 0.52 to 0Показать полностью.65) and the set and ratio of fatty acids (FA), which was represented by acids with chain lengths from C14 to C24. The bacterial biomass concentration and PHA synthesis were comparable (4.1–4.6 g/L and about 70%) when using WFO obtained from smoked-sprat heads and fresh mackerel, and it was twice as high as the bacterial biomass concentration from the fresh sprat waste. This depended on the type of WFO, the bacteria synthesized P(3HB) homopolymer or P(3HB-co-3HV-co-3HHx) copolymer, which had a lower degree of crystallinity (Cx 71%) and a lower molecular weight (Mn 134 kDa) compared to the P(3HB) (Mn 175–209 kDa and Cx 74–78%) at comparable temperatures (Tmelt and Tdegr of 158–168 °C and 261–284 °C, respectively). The new types of WFO, studied for the first time, are suitable as a carbon substrates for PHA synthesis. The WFOs obtained in the production of canned Baltic sprat and Baltic mackerel can be considered a promising and renewable substrate for PHA biosynthesis.</jats:p>
Журнал: International Journal of Molecular Sciences
Выпуск журнала: Т.24, №19
Номера страниц: 14919
ISSN журнала: 16616596
Место издания: Basel
Издатель: Molecular Diversity Preservation International