Тип публикации: статья из журнала
Год издания: 2023
Идентификатор DOI: 10.1002/bio.4499
Аннотация: <jats:title>Abstract</jats:title><jats:p>Bioluminescent bacteria in the form of a cell suspension for on-site hazard analysis are not suitable as <jats:italic>in vivo</jats:italic> luminescence in free cells fluctuates and may lead to erroneous results. Furthermore, the culture broth cannot be stored for long durations to continue Показать полностьюsensing analytes as the luminescence ceases over time. Factors that affect luminescence response include growth dynamism, and ambient environmental conditions. The present study investigated the effect of storage conditions such as temperature (25 ± 2°C, room temperature; 4°C; and −20°C) and ambient aqueous environment (M1: sucrose, 1.02 M; M2, bioluminescent media [tryptone, 10 g L<jats:sup>−1</jats:sup>; NaCl, 28.5 g L<jats:sup>−1</jats:sup>; MgCl<jats:sub>2</jats:sub>.7H<jats:sub>2</jats:sub>O, 4.5 g L<jats:sup>−1</jats:sup>; CaCl<jats:sub>2</jats:sub>, 0.5 g L<jats:sup>−1</jats:sup>; KCl 0.5 g L<jats:sup>−1</jats:sup>; yeast extract, 1 g L<jats:sup>−1</jats:sup>; H<jats:sub>2</jats:sub>O, 1 L]; M3, bioluminescent media and 95% glycerol, 1:1 ratio) on the luminescence emission from the calcium alginate-immobilized <jats:styled-content style="fixed-case"><jats:italic>Photobacterium phosphoreum</jats:italic></jats:styled-content> (S<jats:sub>b</jats:sub>) against the cells in free suspension for an extended period. The results indicated that both the parameters that were undertaken markedly affected the luminescence. In the study, S<jats:sub>b</jats:sub> showed an enhanced luminescence emission than the control up to 18.5-fold and for a prolonged period which can be efficiently utilized for rapid biosensing of hazardous materials.</jats:p>
Журнал: Luminescence
Выпуск журнала: Т. 38, № 6
Номера страниц: 717-721
ISSN журнала: 15227235
Издатель: John Wiley & Sons