Тип публикации: статья из журнала

Год издания: 2023

Идентификатор DOI: 10.3390/technologies11040106

Аннотация: <jats:p>Biodegradable polyhydroxyalkanoates, biopolymers of microbiological origin, formed by 3- and 4-hydroxybutyrate monomers P(3HB-co-4HB), were used to obtain nanomembranes loaded with drugs as cell carriers by electrospinning. Resorbable non-woven membranes from P(3HB-co-4HB) loaded with ceftazidime, doripinem, and actovegin hПоказать полностьюave been obtained. The loading of membranes with drugs differently affected the size of fibers and the structure of membranes, and in all cases increased the hydrophilicity of the surface. The release of drugs in vitro was gradual, which corresponded to the Higuchi and Korsmeyer-Peppas models. Antibiotic-loaded membranes showed antibacterial activity against S. aureus and E. coli, in which growth inhibition zones were 41.7 ± 1.1 and 38.6 ± 1.7 mm for ceftazidime and doripinem, respectively. The study of the biological activity of membranes in the NIH 3T3 mouse fibroblast culture based on the results of DAPI and FITC staining of cells, as well as the MTT test, did not reveal a negative effect despite the presence of antibiotics in them. Samples containing actovegin exhibit a stimulating effect on fibroblasts. Biodegradable polyhydroxyalkanoates formed by 3-hydroxybutyrate and 4-hydroxybutyrate monomers provide electrospinning non-woven membranes suitable for long-term delivery of drugs and cultivation of eukaryotic cells, and are promising for the treatment of wound defects complicated by infection.</jats:p>

Журнал: Technologies

Выпуск журнала: Т.11, №4

Номера страниц: 106

ISSN журнала: 22277080

Издатель: MDPI, ST ALBAN-ANLAGE 66, BASEL, SWITZERLAND, CH-4052

• Volova Tatiana G. (Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi av., 660041 Krasnoyarsk, Russia)
• Demidenko Aleksey V. (Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi av., 660041 Krasnoyarsk, Russia)
• Murueva Anastasiya V. (Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi av., 660041 Krasnoyarsk, Russia)
• Dudaev Alexey E. (Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi av., 660041 Krasnoyarsk, Russia)
• Nemtsev Ivan (L.V. Kirensky Institute of Physics, 50/12 Akademgorodok, 660036 Krasnoyarsk, Russia)
• Shishatskaya Ekaterina I. (Chemistry Engineering Centre, ITMO University, KronverkskiyProspekt, 49A, 197101 Saint Petersburg, Russia)

• Ядро РИНЦ (eLIBRARY.RU)