Тип публикации: статья из журнала
Год издания: 2013
Идентификатор DOI: 10.1021/jm301856j
Ключевые слова: amikacin, ampicillin, antibiotic agent, aptamer, bacterial DNA, chloramphenicol, streptomycin, tetracycline, antibacterial activity, antibiotic resistance, article, bacterial growth, bactericidal activity, bacteriostasis, bacterium culture, cell membrane depolarization, dissociation constant, DNA sequence, drug binding site, drug DNA binding, human, membrane potential, nonhuman, Salmonella enteritidis, Salmonella typhimurium, serotype, Anti-Bacterial Agents, Aptamers, Nucleotide, Base Sequence, Drug Resistance, Multiple, Bacterial, Molecular Sequence Data, SELEX Aptamer Technique, Structure-Activity Relationship
Аннотация: Salmonella is one of the most dangerous and common food-borne pathogens. The overuse of antibiotics for disease prevention has led to the development of multidrug resistant Salmonella. Now, more than ever, there is a need for new antimicrobial drugs to combat these resistant bacteria. Aptamers have grown in popularity since their dПоказать полностьюiscovery, and their properties make them attractive candidates for therapeutic use. In this work, we describe the selection of highly specific DNA aptamers to S. enteritidis and S. typhimurium. To evolve species specific aptamers, twelve rounds of selection to live S. enteritidis and S. typhimurium were performed, alternating with a negative selection against a mixture of related pathogens. Studies have shown that synthetic pools combined from individual aptamers have the capacity to inhibit growth of S. enteritidis and S. typhimurium in bacterial cultures; this was the result of a decrease in their membrane potential.
Журнал: JOURNAL OF MEDICINAL CHEMISTRY
Выпуск журнала: Vol. 56, Is. 4
Номера страниц: 1564-1572
ISSN журнала: 00222623
Место издания: WASHINGTON
Издатель: AMER CHEMICAL SOC