Тип публикации: статья из журнала
Год издания: 2022
Идентификатор DOI: 10.1038/s41598-022-24117-5
Аннотация: <jats:title>Abstract</jats:title><jats:p>Coelenterazine-<jats:italic>v</jats:italic> (CTZ-<jats:italic>v</jats:italic>), a synthetic vinylene-bridged π-extended derivative, is able to significantly alter bioluminescence spectra of different CTZ-dependent luciferases and photoproteins by shifting them towards longer wavelengths. HowПоказать полностьюever, Ca<jats:sup>2+</jats:sup>-regulated photoproteins activated with CTZ-<jats:italic>v</jats:italic> display very low bioluminescence activities that hampers its usage as a substrate of photoprotein bioluminescence. Here, we report the crystal structure of semi-synthetic Ca<jats:sup>2+</jats:sup>-discharged obelin-<jats:italic>v</jats:italic> bound with the reaction product determined at 2.1 Å resolution<jats:italic>.</jats:italic> Comparison of the crystal structure of Ca<jats:sup>2+</jats:sup>-discharged obelin-<jats:italic>v</jats:italic> with those of other obelins before and after bioluminescence reaction reveals no considerable changes in the overall structure. However, the drastic changes in CTZ-binding cavity are observed owing to the completely different reaction product, coelenteramine-<jats:italic>v</jats:italic> (CTM-<jats:italic>v</jats:italic>). Since CTM-<jats:italic>v</jats:italic> is certainly the main product of obelin-<jats:italic>v</jats:italic> bioluminescence and is considered to be a product of the “dark” pathway of dioxetanone intermediate decomposition, it explains the low bioluminescence activity of obelin and apparently of other photoproteins with CTZ-<jats:italic>v</jats:italic>.</jats:p>
Журнал: Scientific Reports
Выпуск журнала: Т.12, №1
Номера страниц: 19613
ISSN журнала: 20452322