Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций
Конференция: XVI International Conference on Pulsed Lasers and Laser Applications; Tomsk; Tomsk
Год издания: 2023
Идентификатор DOI: 10.1117/12.3009717
Ключевые слова: fluorescein, exited state proton transfer, ionic equilibrium, kinetic approach, fluorescence, absorbance, ratiometric signal
Аннотация: In the work, the priority among a number of temperature mechanisms affecting the fluorescence ratiometric signal of fluorescein in the pH range of 5.5÷7 was revealed. The kinetic model at the monoanion-dianion dissociation step with temperature-dependent constants was applied to analyze the experimental data. It was shown that the Показать полностьюmajor contribution to the sensitivity of the ratiometric signal on temperature provides the perturbation of the ionic equilibrium in the ground state. The thermometric characteristics of ratiometric signal were found and the temperature resolution of 7 o C was achieved. Temperature resolution can be improved up to 2oC by optimization of the experimental conditions.
Журнал: XVI International Conference on Pulsed Lasers and Laser Applications
Номера страниц: 129200-129200
Место издания: Bellingham