Тип публикации: статья из журнала

Год издания: 2023

Ключевые слова: enzyme inhibition-based assay, pesticides, bioluminescent assay, luminous bacteria, conjugated enzyme reactions, formulants

Аннотация: Pesticides can affect the health of individual organisms and the function of the entire ecosystem. Therefore, thorough assessment of the risks associated with the use of pesticides is a high-priority task. An enzyme inhibition-based assay is used in this study as a convenient and quick tool to study the effects of pesticides at theПоказать полностьюmolecular level. The contribution of formulants to toxicological properties of the pesticide formulations has been studied by analyzing effects of 7 active ingredients of pesticides (AIas) and 10 commercial formulations based on them (AIfs) on the function of a wide range of enzyme assay systems differing in complexity (single-, coupled, and three-enzyme assay systems). Results have been compared with the effects of AIas and AIfs on bioluminescence of the luminous bacterium Photobacterium phosphoreum. Mostly, AIfs produce a considerably stronger inhibitory effect on the activity of enzyme assay systems and bioluminescence of the luminous bacterium than AIas, which confirms the contribution of formulants to toxicological properties of the pesticide formulation. Results of the current study demonstrate that “inert” ingredients are not ecotoxicologically safe and can considerably augment the inhibitory effect of pesticide formulations; therefore, their use should be controlled more strictly. Circular dichroism and fluorescence spectra of the enzymes used for assays do not show any changes in the protein structure in the presence of commercial pesticide formulations during the assay procedure. This finding suggests that pesticides produce the inhibitory effect on enzymes through other mechanisms.

Журнал: International Journal of Molecular Sciences

Выпуск журнала: Т. 24, № 3

Номера страниц: 2248

ISSN журнала: 16616596

Место издания: Basel

Издатель: Molecular Diversity Preservation International?

• Esimbekova Elena N. (Institute of Biophysics of Siberian Branch of Russian Academy of Science)
• Kalyabina Valeriya P. (Institute of Biophysics of Siberian Branch of Russian Academy of Science)
• Kopylova Kseniya V. (Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science)
• Lonshakova-Mukina Victoria I. (Institute of Biophysics of Siberian Branch of Russian Academy of Science)
• Antashkevich Anna A. (Siberian Federal University)
• Torgashina Irina G. (Siberian Federal University)
• Lukyanenko Kirill A. (Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University)
• Nemtseva Elena V. (Institute of Biophysics of Siberian Branch of Russian Academy of Science)
• Kratasyuk Valentina A. (Institute of Biophysics of Siberian Branch of Russian Academy of Science)

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