Тип публикации: статья из журнала

Год издания: 2022

Идентификатор DOI: 10.1134/s1062359022050193

Ключевые слова: somatic embryogenesis, plant regeneration, clones, genotyping, Larix, life sciences, general, cell biology, biochemistry, zoology, ecology

Аннотация: The results of a study of the regenerative capacity of eleven cell lines (CLs) of Siberian larch cultivated in vitro on AI nutrient medium for one to ten years are presented. The CLs differed in the productivity and ploidy of embryogenic cultures, the morphogenesis of somatic embryos, and their ability to maturate and germinate. A Показать полностьюhigh regenerative capacity was found in CL6. Embryogenic cultures of this cell line produced large globular embryos and had a high productivity of the embryonal-suspensor mass (ESM) and stable ploidy. Genotyping at nine nuclear microsatellite loci of cloned larch trees showed complete identity of their CL6, from which they were obtained. At the age of seven, generative organs were formed in tree clones. In the subsequent spring–summer period, the process of micro- and macrosporogenesis was completed and pollination and seed development took place.

Журнал: Biology Bulletin

Выпуск журнала: Т. 49, № 6

Номера страниц: 609-619

ISSN журнала: 10623590

Место издания: Moscow

Издатель: Pleiades Publishing, Inc.

• Tretyakova I.N. (Sukachev Institute of Forests, Federal Research Center, Krasnoyarsk Science Center, Russian Academy of Sciences, Siberian Branch,”)
• Park M.E. (Sukachev Institute of Forests, Federal Research Center, Krasnoyarsk Science Center, Russian Academy of Sciences, Siberian Branch,”)
• Oreshkova N.V. (Genome Research and Education Center, Siberian Federal University)
• Padutov V.E. (Forest Research Institute of National Academy of Sciences of Belarus)

• Ядро РИНЦ (eLIBRARY.RU)