Тип публикации: статья из журнала

Год издания: 2023

Идентификатор DOI: 10.3389/fmolb.2023.1184285

Ключевые слова: RNA-aptamer, Mda-mb-231, breast cancer, molecular subtypes, circulating tumor cells, mammaglobin

Аннотация: Introduction: Breast cancer (BC) diagnostics lack noninvasive methods and procedures for screening and monitoring disease dynamics. Admitted CellSearch® is used for fluid biopsy and capture of circulating tumor cells of only epithelial origin. Here we describe an RNA aptamer (MDA231) for detecting?BC cells in clinical samples, inclПоказать полностьюuding blood. The MDA231 aptamer was originally selected against triple-negative breast cancer cell line MDA-MB-231 using cell- SELEX.?Methods: The aptamer structure in solution was predicted using mFold program and molecular dynamic simulations. The affinity and specificity of the evolved aptamers were evaluated by flow cytometry and laser scanning microscopy on clinical tissues from breast cancer patients. CTCs were isolated form the patients' blood using the developed method of aptamer-based magnetic separation. Breast cancer origin of CTCs was confirmed by cytological, RT-qPCR and?Immunocytochemical analyses.?Results: MDA231 can specifically recognize breast cancer cells in surgically resected tissues from patients with different molecular subtypes: triple-negative,Luminal A, and Luminal B, but not in benign tumors, lung cancer, glial tumor and healthy epithelial from lungs and breast. This RNA aptamer can identify cancer cells in complex cellular environments, including tumor biopsies (e.g., tumor tissues vs.?margins) and clinical blood samples (e.g., circulating tumor cells). Breast cancer origin of the aptamer-based magnetically separated CTCs has been proved by immunocytochemistry and mammaglobin mRNA expression.?Discussion: We suggest a simple, minimally-invasive breast cancer diagnostic method based on non-epithelial MDA231 aptamer-specific magnetic isolation of circulating tumor cells. Isolated cells are intact and can be utilized for molecular diagnostics purposes.?

Журнал: Frontiers in Molecular Biosciences

Выпуск журнала: Т.10

Номера страниц: 1184285

ISSN журнала: 2296889X

Место издания: Лозанна

• Kolovskaya Olga S. (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of?Science”)
• Zyuzyukina Alena V. (Krasnoyarsk Regional?Clinical Cancer Center Named After A.I. Kryzhanovsky)
• Dassie Justin P. (University of Iowa)
• Zamay Galina S. (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of?Science”)
• Zamay Tatiana N. (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of?Science”)
• Boyakova Nina V. (Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University)
• Khorzhevskii Vladimir A. (Krasnoyarsk Regional Pathology-?Anatomic Bureau)
• Kirichenko Daria A. (Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University)
• Lapin Ivan N. (Siberian?Physical Technical Institute, Tomsk State University)
• Shchugoreva Irina A. (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of?Science”)
• Artyushenko Polina V. (Siberian Federal University)
• Tomilin Felix N. (Kirensky Institute of Physics)
• Veprintsev Dmitry V. (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of?Science”)
• Glazyrin Yury E. (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of?Science”)
• Zoran Minic (University of Ottawa)
• Bozhenko Vladimir K. (Russian Scientific Center of?Roentgenoradiology)
• Kudinova Elena A. (Russian Scientific Center of?Roentgenoradiology)
• Kiseleva Yana Y. (Russian Scientific Center of?Roentgenoradiology)
• Krat Alexey V. (Krasnoyarsk Regional?Clinical Cancer Center Named After A.I. Kryzhanovsky)
• Slepov Eugene V. (Krasnoyarsk Regional?Clinical Cancer Center Named After A.I. Kryzhanovsky)
• Bukatin Anton S. (Institute for Analytical Instrumentation of the Russian Academy of?Sciences)
• Zukov Ruslan A. (Krasnoyarsk Regional?Clinical Cancer Center Named After A.I. Kryzhanovsky)
• Shesternya Pavel A. (Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University)
• Berezovski Maxim V. (University of Ottawa)
• Giangrande Paloma H. (latform Discovery Sciences, Biology, Wave Life Sciences)
• Kichkailo Anna S. (Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of?Science”)

• Ядро РИНЦ (eLIBRARY.RU)