Biocompatibility of polyhydroxybutyrate microspheres: in vitro and in vivo evaluation


Тип публикации: статья из журнала

Год издания: 2008

Идентификатор DOI: 10.1007/s10856-007-3345-6

Ключевые слова: Biocompatibility, Drug delivery, Fibroblasts, Ion implantation, Microspheres, Polymer matrix, Fibroblast cells, Hydroxybutyric acid, Intramuscular implantation, Polyhydroxybutyrate, Organic polymers, microsphere, poly(3 hydroxybutyric acid), polymer, animal cell, animal experiment, animal tissue, article, cell infiltration, controlled study, giant cell, implantation, in vitro study, in vivo study, inflammation, macrophage, mouse, nonhuman, priority journal, rat, 3-Hydroxybutyric Acid, Animals, Biocompatible Materials, Cell Survival, Materials Testing, Mice, Necrosis, NIH 3T3 Cells, Polymers, Rats, Rats, Wistar, Tetrazolium Salts, Thiazoles, Time Factors, Rattus norvegicus

Аннотация: Microspheres have been prepared from the resorbable linear polyester of beta-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experimentsПоказать полностьюon intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection.

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Выпуск журнала: Vol. 19, Is. 6

Номера страниц: 2493-2502

ISSN журнала: 09574530

Место издания: DORDRECHT

Издатель: SPRINGER


  • Shishatskaya Ekaterina Igorevna (SB RAS, Inst Biophys, Lab Chemoautotroph Biosynth, Krasnoyarsk 660036, Russia)
  • Voinova Olga N. (SB RAS, Inst Biophys, Lab Chemoautotroph Biosynth, Krasnoyarsk 660036, Russia)
  • Goreva Anastasiya V. (SB RAS, Inst Biophys, Lab Chemoautotroph Biosynth, Krasnoyarsk 660036, Russia)
  • Mogilnaya Olga A. (SB RAS, Inst Biophys, Lab Bacterial Bioluminescence, Krasnoyarsk 660036, Russia)
  • Volova Tatiana G. (SB RAS, Inst Biophys, Lab Chemoautotroph Biosynth, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia)