Тип публикации: статья из журнала
Год издания: 2009
Идентификатор DOI: 10.1134/S1021443709050100
Ключевые слова: Larix sibirica, immune-enzyme analysis, zygotic embryo, endosperm, androgenesis in vitro, morphogenic callus, embryoid
Аннотация: Androgenesis in vitro in plants is a phenomenon of developmental switching of male generative cells, microspores, from their normal gametophytic to sporophytic pathway. We obtained androgenic callus and embryoids (embryo-like structures derived from microspores) of the conifer plant, Siberian larch (Larix sibirica Ledeb.) in the inПоказать полностьюvitro culture. The immune-enzyme analysis of the hormonal balance of larch androgenic cultures showed a substantial increase in the content of all phytohormones, especially cytokinins and ABA, as compared with initial explants (microstrobiles). This was evidently related to active cell divisions and embryoid formation. A comparison of androgenic cultures derived from trees nonifested and infested with larch gall midges (Dasineura rozhkovi Main. et Nik.) revealed a cytokinin content increase (by two times) and an ABA content decrease (by two times), which indicates more intense growth of cultures derived from healthy trees. Phytohormone content in the androgenic callus was compared with their accumulation in the embryos of larch seeds harvested from noninfested trees. We concluded that successful growth of androgenic cultures and embryoid formation demand an additional medium supplement with auxins (not more than 0.5 mg/l).
Журнал: RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
Выпуск журнала: Vol. 56, Is. 5
Номера страниц: 647-653
ISSN журнала: 10214437
Место издания: NEW YORK
Издатель: MAIK NAUKA/INTERPERIODICA/SPRINGER