Development of Cellular and Enzymatic Bioluminescent Assay Systems to Study Low-Dose Effects of Thorium : научное издание

Описание

Тип публикации: статья из журнала

Год издания: 2021

Идентификатор DOI: 10.3390/bioengineering8120194

Ключевые слова: bioassay, bioluminescence, luminous bacteria, enzymes, reactive oxygen species, thorium, low-dose exposure, radiation hormesis

Аннотация: Thorium is one of the most widespread radioactive elements in natural ecosystems, along with uranium, it is the most important source of nuclear energy. However, the effects of thorium on living organisms have not been thoroughly studied. Marine luminescent bacteria and their enzymes are optimal bioassays for studying low-dose thorПоказать полностьюium exposures. Luminescent bioassays provide a quantitative measure of toxicity and are characterized by high rates, sensitivity, and simplicity. It is known that the metabolic activity of bacteria is associated with the production of reactive oxygen species (ROS). We studied the effects of thorium-232 (10(-11)-10(-3) M) on Photobacterium phosphoreum and bacterial enzymatic reactions; kinetics of bacterial bioluminescence and ROS content were investigated in both systems. Bioluminescence activation was revealed under low-dose exposures (

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Издание

Журнал: BIOENGINEERING-BASEL

Выпуск журнала: Vol. 8, Is. 12

Номера страниц: 194

Место издания: MDPI

Издатель: 2

Персоны

  • Kolesnik Olga V. (RAS, Krasnoyarsk Sci Ctr SB, Inst Biophys SB, Fed Res Ctr, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia)
  • Rozhko Tatiana V. (Krasnoyarsk State Med Acad, Krasnoyarsk 660022, Russia)
  • Lapina Maria A. (Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia)
  • Solovyev Vladislav S. (Natl Res Tomsk Polytech Univ, Tomsk 634050, Russia)
  • Sachkova Anna S. (Natl Res Tomsk Polytech Univ, Tomsk 634050, Russia)
  • Kudryasheva Nadezhda S. (RAS, Krasnoyarsk Sci Ctr SB, Inst Biophys SB, Fed Res Ctr, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia)