Тип публикации: статья из журнала
Год издания: 2022
Идентификатор DOI: 10.1002/jssc.202200453
Ключевые слова: drug delivery, host-guest complex, inclusion complex, pentacyclic lupane triterpenoids, solubilization
Аннотация: The values of the apparent binding constants for β-cyclodextrin complexes ofbetulin derivatives determined by mobility shift affinity capillary electrophoresiswere found to be independent of the composition of the two background elec-trolytes used (tetraborate buffer, pH 9.18, and phosphate buffer, pH 8.00, both ofthem with 20 mM iПоказать полностьюonic strength). It has been found that if there is not a con-stant plateau on the binding curve then four independent parameters can bedetermined: binding constants (also referred to as stability, association, or for-mation constants) and ionic mobilities of 1:1 and 1:2 complexes. However, at least10–12 data points in the binding curve should be used to reliably estimate theparameters. For the first time, the apparent binding constants for complexes ofester betulin derivatives with dimethyl-β-cyclodextrin have been determined bymobility shift affinity capillary electrophoresis. The logarithms of the constantsfor1:1and1:2complexesat25◦C for betulin 3,28-diphthalate with a 95% confi-dence interval are 4.98 (4.95–5.01) and 7.52 (7.26–7.68); for betulin 3,28-disulfate,the values are 4.97 (4.89–5.03) and 8.24 (6.82–8.52). It has been found that betulin3,28-disuccinate forms only a 1:1 complex and the binding constant logarithm is5.25±0.02.
Журнал: Journal of Separation Science
Выпуск журнала: Т. 45, № 19
Номера страниц: 3745-3753
ISSN журнала: 16159306
Издатель: Wiley Interscience