Тип публикации: статья из журнала
Год издания: 2020
Идентификатор DOI: 10.1002/bio.3954
Ключевые слова: athletes, bluc‐, red coupled enzyme system, catalase activity, saliva, training load, bluc-red coupled enzyme system
Аннотация: Upcoming professional sports authorities seek rapid noninvasive biosensing tools for regular monitoring of athletes' physiological states. The analysis of saliva through luminescence-based biosensors has been perceived as a suitable candidate for such purposes. The present study reports a qualitative bioluminescence assay based on Показать полностьюa coupled enzyme system that consists of bacterial luciferase (BLuc) and nicotinamide adenine dinucleotide (NADH):flavin mononucleotide (FMN) oxidoreductase (Red), BLuc-Red, for the express diagnostics of athletes' stress levels before and after physical exertion. The volunteers who participated in the study were grouped as freestyle wrestlers and students who adapted to different levels of physical activities. Under physical exertion modelling conditions, the influence of participant saliva on BLuc-Red catalyzed light emission was investigated. Results showed a significant increase in residual luminescence (I-exp, mean maximum bioluminescence intensity of the experimental measurement (I-exp); I-c, luminescence intensity in control; I-exp/I-c, %) values for participants in the wrestler group while a decrease in the student group (P
Журнал: LUMINESCENCE
Выпуск журнала: Is. 2
ISSN журнала: 15227235
Место издания: HOBOKEN
Издатель: WILEY