In vitro and transdermal penetration of PHBV micro/nanoparticles


Тип публикации: статья из журнала

Год издания: 2014

Идентификатор DOI: 10.1007/s10856-014-5169-5

Ключевые слова: Cell penetration, Polymeric micro/nanospheres, Transdermal drug delivery, Cell culture, Cell membranes, Emulsions, Gas chromatography, Gene transfer, Polymethyl methacrylates, Skin, Gas chromatography-mass spectrometry, Nano-sized drug carriers, Poly(3-hydroxybutyrate-co-3-hydroxyvalerate), Polydispersity indices, Transdermal permeation, Dermatology, fluorescent dye, microsphere, nanoparticle, nanosphere, poly(3 hydroxybutyrate co 3 hydroxyvalerate), polymer, unclassified drug, animal cell, animal experiment, animal tissue, article, cell membrane, cell proliferation, cell viability, controlled study, cytotoxicity, drug delivery system, emulsion, fibroblast, histopathology, in vitro study, mass fragmentography, mouse, nonhuman, particle size, priority journal, scanning electron microscopy, skin irritation, skin penetration, Absorption, Administration, Cutaneous, Animals, Capsules, Cell Line, Cell Survival, Fibroblasts, Materials Testing, Mice, Mice, Inbred BALB C, Nanocapsules, Polyesters, Skin Absorption, Tissue Distribution

Аннотация: The purpose of this study was to develop micro and nano sized drug carriers from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and study the cell and skin penetration of these particles. PHBV micro/nanospheres were prepared by o/w emulsion method and were stained with a fluorescent dye, Nile Red. The particles were fractionaПоказать полностьюted by centrifugation to produce different sized populations. Topography was studied by SEM and average particle size and its distribution were determined with particle sizer. Cell viability assay (MTT) was carried out using L929 fibroblastic cell line, and particle penetration into the cells were studied. Transdermal permeation of PHBV micro/nanospheres and tissue reaction were studied using a BALB/c mouse model. Skin response was evaluated histologically and amount of PHBV in skin was determined by gas chromatography-mass spectrometry. The average diameters of the PHBV micro/nanosphere batches were found to be 1.9 mu m, 426 and 166 nm. Polydispersity indices showed that the size distribution of micro sized particles was broader than the smaller ones. In vitro studies showed that the cells had a normal growth trend. MTT showed no signs of particle toxicity. The 426 and 166 nm sized PHBV spheres were seen to penetrate the cell membrane. The histological sections revealed no adverse effects. In view of this data nano and micro sized PHBV particles appeared to have potential to serve as topical and transdermal drug delivery carriers for use on aged or damaged skin or in cases of skin diseases such as psoriasis, and may even be used in gene transfer to cells.

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Выпуск журнала: Vol. 25, Is. 6

Номера страниц: 1471-1481

ISSN журнала: 09574530

Место издания: DORDRECHT

Издатель: SPRINGER


  • Eke G. (Department of Chemistry, Faculty of Arts and Sciences, Ahi Evran University)
  • Hasirci N. (Department of Chemistry, Middle East Technical University)
  • Hasirci V. (Department of Biological Sciences, Middle East Technical University)
  • Kuzmina A.M. (Siberian Federal University)
  • Shishatskaya E.I. (Institute of Biophysics SB RAS)
  • Goreva A.V. (Institute of Biophysics SB RAS)