Тип публикации: статья из журнала

Год издания: 2021

Идентификатор DOI: 10.1016/j.snb.2020.128991

Ключевые слова: binding kinetics, fet, imatinib, LMW drugs, single-crystal graphene

Аннотация: We synthesized large-area single-crystal graphene sheets to use them in biosensors based on field-effect transistors (FET) for quantitative analysis of interaction kinetics and affinity between the imatinib drug and its target protein kinase Abl1. The G-FET biosensor showed an excellent performance and recognized imatinib at as low as 15.5 fM. The biosensor also showed a linear response to the logarithm of imatinib concentration in the 0.1 pM-10 μM range. This graphene-based FET biosensor (G-FET) was also applied to quantify Abl1 Y253 F mutation and Abl1 dependency on Mg²⁺ to bind to imatinib in real-time. Results demonstrated in this work clearly showed that the novel G-FET biosensors are very promising to analyze interactions between proteins and low molecular weight drugs.

Журнал: Sensors and Actuators B: Chemical

Выпуск журнала: Т. 326

Номера страниц: 128991

ISSN журнала: 09254005

Издатель: Elsevier Science Publishing Company, Inc.

• Xu S. (Shandong Key Laboratory of Biophysics,Institute of Biophysics,Dezhou University)
• Wang T. (Shandong Key Laboratory of Biophysics,Institute of Biophysics,Dezhou University)
• Liu G. (Shandong Key Laboratory of Biophysics,Institute of Biophysics,Dezhou University)
• Cao Z. (Shandong Key Laboratory of Biophysics,Institute of Biophysics,Dezhou University)
• Li Z. (Shandong Key Laboratory of Biophysics,Institute of Biophysics,Dezhou University)
• Li Q. (Shandong Key Laboratory of Biophysics,Institute of Biophysics,Dezhou University)
• Sha Y. (Shandong Key Laboratory of Biophysics,Institute of Biophysics,Dezhou University)
• Zhang X. (Shandong Key Laboratory of Biophysics,Institute of Biophysics,Dezhou University)
• Liu H. (Shandong Key Laboratory of Biophysics,Institute of Biophysics,Dezhou University)
• Wang J. (Shandong Key Laboratory of Biophysics,Institute of Biophysics,Dezhou University)
• Frank L.A. (Institute of Biophysics SB RAS,Federal Research Center “Krasnoyarsk Science Center SB RAS”)
• Krasitskaya V.V. (Institute of Biophysics SB RAS,Federal Research Center “Krasnoyarsk Science Center SB RAS”)
• Jiang S. (Collaborative Innovation Center of Light Manipulations and Applications,Shandong Normal University)
• Zhang C. (Collaborative Innovation Center of Light Manipulations and Applications,Shandong Normal University)

• Ядро РИНЦ (eLIBRARY.RU)