Тип публикации: статья из журнала
Год издания: 2021
Идентификатор DOI: 10.1016/j.snb.2020.128991
Ключевые слова: binding kinetics, fet, imatinib, LMW drugs, single-crystal graphene
Аннотация: We synthesized large-area single-crystal graphene sheets to use them in biosensors based on field-effect transistors (FET) for quantitative analysis of interaction kinetics and affinity between the imatinib drug and its target protein kinase Abl1. The G-FET biosensor showed an excellent performance and recognized imatinib at as low as 15.5 fM. The biosensor also showed a linear response to the logarithm of imatinib concentration in the 0.1 pM-10 μM range. This graphene-based FET biosensor (G-FET) was also applied to quantify Abl1 Y253 F mutation and Abl1 dependency on Mg2+ to bind to imatinib in real-time. Results demonstrated in this work clearly showed that the novel G-FET biosensors are very promising to analyze interactions between proteins and low molecular weight drugs.
Журнал: Sensors and Actuators B: Chemical
Выпуск журнала: Т. 326
Номера страниц: 128991
ISSN журнала: 09254005
Издатель: Elsevier Science Publishing Company, Inc.