The Effect of Osmolytes on the Bioluminescent Reaction of Bacteria: Structural and Dynamic Properties

Описание

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1134/S0006350920060202

Ключевые слова: bacterial luciferase, bioluminescence, luminous bacteria, molecular dynamic, osmolyte, protein structure and dynamics

Аннотация: The effects of viscous media with glycerol and sucrose (10–40%) on the kinetics of the bacterial bioluminescent reaction have been investigated by stopped-flow technique. Increment of quantum yield in media with 10% of both osmolytes was shown. Higher concentrations of glycerol, up to 30–40%, were found to reduce the efficiency of Показать полностьюthe reaction, while this effect was not observed in the media with sucrose. The molecular dynamics simulation was used to study the structure of bacterial luciferase surrounded by either water molecules solely or by mixture of water with various numbers of glycerol/sucrose molecules. It was found that both cosolvents at studied concentrations did not cause a significant change in conformation of bacterial luciferase. The calculated root-mean-square fluctuation for Cα-atoms of bacterial luciferase α-subunit indicated that the higher flexibility of the enzyme mobile loop could be responsible for increment of quantum yield in the presence of 10% of both osmolytes. The active site of bacterial luciferase was found to be accessible for glycerol molecules while sucrose did not enter catalytic gorge. Moreover, at 30 and 40% concentration the glycerol molecules were found to locate in the active site of bacterial luciferase throughout the whole simulation time. © 2020, Pleiades Publishing, Inc.

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Издание

Журнал: Biophysics (Russian Federation)

Выпуск журнала: Vol. 65, Is. 6

Номера страниц: 966-971

ISSN журнала: 00063509

Издатель: Pleiades journals

Авторы

  • Sukovatyi L.A. (Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)
  • Lisitsa A.E. (Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)
  • Kratasyuk V.A. (Siberian Federal University, Krasnoyarsk, 660041, Russian Federation, Institute of Biophysics SB RAS, Krasnoyarsk, 660036, Russian Federation)
  • Nemtseva E.V. (Siberian Federal University, Krasnoyarsk, 660041, Russian Federation, Institute of Biophysics SB RAS, Krasnoyarsk, 660036, Russian Federation)

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