Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций
Конференция: Теоретические и прикладные аспекты биохимии и биотехнологии растений : III Международная научная конференция; Минск; Минск
Год издания: 2008
Аннотация: A DNA fragment, encoding thaumatin-like protein (TLP) from kiwifruit ( Actinidia deliciosa ) was cloned into the binary vector pBINmGFP5ER under the control of the CaMV 35S promoter for agrobacterium-mediated transformation of Nicotiana tobacum. All putative transformants were tested for the integration of the kanamycin resistance Показать полностьюgene ( nptII ) and kiwifruit TLP ( ktlp ) gene by PCR. The expression of kiwifruit TLP gene was observed in transgenic plants using RT-PCR analysis. In future research we plan to study the expression of the protein in transgenic tobacco plants and to analyze resistance of lines expressing kiwifruit TLP to pathogenic fungi. A DNA fragment, encoding thaumatin-like protein (TLP) from kiwifruit ( Actinidia deliciosa ) was cloned into the binary vector pBINmGFP5ER under the control of the CaMV 35S promoter for agrobacterium-mediated transformation of Nicotiana tobacum. All putative transformants were tested for the integration of the kanamycin resistance gene ( nptII ) and kiwifruit TLP ( ktlp ) gene by PCR. The expression of kiwifruit TLP gene was observed in transgenic plants using RT-PCR analysis. In future research we plan to study the expression of the protein in transgenic tobacco plants and to analyze resistance of lines expressing kiwifruit TLP to pathogenic fungi.
Журнал: Теоретические и прикладные аспекты биохимии и биотехнологии растений
Номера страниц: 167-171
Издатель: Белорусский государственный университет