Sequence Capture of Mitochondrial Genome with PCR-Generated Baits Provides New Insights into the Biogeography of the Genus Abies Mill

Описание

Тип публикации: статья из журнала

Год издания: 2022

Идентификатор DOI: 10.3390/plants11060762

Ключевые слова: intercontinental migrations, mitochondrial dna, molecular dating, phylogeny, target enrichment

Аннотация: Mitochondrial DNA (mtDNA), being maternally inherited in plants of the family Pinaceae, is an important source of phylogeographic information. However, its use is hindered by a low mutation rate and frequent structure rearrangements. In the present study, we tested the method of genomic libraries enrichment with mtDNA via the sequeПоказать полностьюnce capture method yielding mtDNA data which were further used to reconstruct the phylogenetic tree of the genus Abies. The baits for hybrid capture were obtained by long-range PCR using primers designed on the basis of the assembly of Abies sibirica Ledeb. mitochondrial genome. Mitochondrial genomes of Picea sitchensis (Bong.) Carr., Larix sibirica Ledeb., and Keteleeria davidiana (Bertrand) Beissn. were used as an outgroup. The resulting phylogenetic tree consists of two sister branches, including the Eurasian and American species, respectively, with some exceptions. The subclade of A. sachalinensis (F. Schmidt) Mast. and A. veitchii Lindl. (Japan and Sakhalin islands) occupies a basal position in the branch of American firs, probably due to the complex history of fir migrations from North America to Eurasia. The tree has high support for majority of clades. For species represented by more than one sample an intraspecific variability was found which is suitable to design mtDNA markers for phylogeographic and population studies. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

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Издание

Журнал: Plants

Выпуск журнала: Vol. 11, Is. 6

Номера страниц: 762

Персоны

  • Semerikov V.L. (Institute of Plant and Animal Ecology, Ural Branch of the Russian Academy of Sciences, Ekaterinburg, 620144, Russian Federation)
  • Semerikova S.A. (Institute of Plant and Animal Ecology, Ural Branch of the Russian Academy of Sciences, Ekaterinburg, 620144, Russian Federation)
  • Khrunyk Y.Y. (Department of Heat Treatment and Physics of Metal, Ural Federal University Named after the First President of Russia B.N. Yeltsin, Ekaterinburg, 620002, Russian Federation)
  • Putintseva Y.A. (Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)