Enzymatic responses to low-intensity radiation of tritium

Описание

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.3390/ijms21228464

Ключевые слова: bacterial luciferase, enzymes, fluorescent protein, hormesis, low-dose radiation, oxidoreductase, tritium

Аннотация: The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reaПоказать полностьюctions, were studied at radioactivity concentrations ≤ 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (

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Издание

Журнал: International Journal of Molecular Sciences

Выпуск журнала: Vol. 21, Is. 22

Номера страниц: 1-15

ISSN журнала: 16616596

Издатель: MDPI AG

Авторы

  • Rozhko Tatiana V. (Krasnoyarsk State Med Acad, Dept Med & Biol Phys, Krasnoyarsk 660022, Russia)
  • Nemtseva Elena V. (Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia; RAS, Inst Biophys, SB, FRC,KSC, Krasnoyarsk 660036, Russia)
  • Gardt Maria V. (Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia)
  • Raikov Alexander V. (Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia)
  • Lisitsa Albert E. (Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia)
  • Badun Gennadii A. (Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia)
  • Kudryasheva Nadezhda S. (Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia; RAS, Inst Biophys, SB, FRC,KSC, Krasnoyarsk 660036, Russia)

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