Bioluminescent enzyme inhibition-based assay for the prediction of toxicity of pollutants in urban soils

Описание

Тип публикации: статья из журнала

Год издания: 2021

Идентификатор DOI: 10.1016/j.eti.2021.101842

Ключевые слова: bioassay, bioluminescence, industrial contamination, soil pollution, urbostratozems

Аннотация: There is a need for rapid simple and informative environmental assessment methods. The present investigation is aimed at assessing the possibility of using the combined enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc) for predicting the potential toxicity of industrial urbostratozems sampleПоказать полностьюd in the city of Krasnoyarsk. Three groups of urbostratozems polluted with fluorine, arsenic and lead, were tested by the methods of chemical analysis and enzymatic bioassay. Only the assessment of the arsenic-contaminated soil samples showed the dependence between the reduced activity of the enzyme system and the arsenic concentration variations. The results reveal that the sensitivity of the Red + Luc enzyme system to the soil pollutants depends on the properties of the studied soil samples. Moreover, the solubility of lead in the soil samples affects the accuracy of the enzymatic bioassays for soil toxicity testing. The results of the enzymatic bioassay of the fluoride-contaminated soil samples are ambiguous. The obtained data show the relevance of the sample preparation during integral bioassays. In addition, soil properties should be taken into account as well. The current study emphasizes the importance of conducting chemical and biological testing as a combined set to obtain comprehensive information about the anthropogenic load. © 2021 Elsevier B.V.

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Издание

Журнал: Environmental Technology and Innovation

Выпуск журнала: Vol. 24

Номера страниц: 101842

ISSN журнала: 23521864

Издатель: Elsevier B.V.

Персоны

  • Kolosova Elizaveta M. (Siberian Fed Univ, Dept Biophys, 79 Svobodny St, Krasnoyarsk 660041, Russia)
  • Sutormin Oleg S. (Siberian Fed Univ, Dept Biophys, 79 Svobodny St, Krasnoyarsk 660041, Russia)
  • Stepanova Ludmila V. (Department of Biophysics, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)
  • Shpedt Aleksandr A. (Russian Acad Sci, Krasnoyarsk Agr Res Inst, Fed Res Ctr Krasnoyarsk Sci Ctr Siberian Branch, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Dept Aquat & Terr Ecosyst, Krasnoyarsk 660041, Russia)
  • Rimatskaya Nadezhda V. (Department of Biophysics, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)
  • Sukovataya Irina E. (Siberian Fed Univ, Dept Biophys, 79 Svobodny St, Krasnoyarsk 660041, Russia)
  • Kratasyuk Valentina A. (Siberian Fed Univ, Dept Biophys, 79 Svobodny St, Krasnoyarsk 660041, Russia; Russian Acad Sci, Photobiol Lab, Inst Biophys, Fed Res Ctr `Krasnoyarsk Sci Ctr Siberian Branch, Krasnoyarsk 660036, Russia)