Biological activity of carbonic nano-structures-comparison via enzymatic bioassay : научное издание


Тип публикации: статья из журнала

Год издания: 2018

Ключевые слова: antioxidant activity, bioactive compounds, fullerenol, humic substances, reactive oxygen species, toxicity

Аннотация: Purpose: The aim of the work is to compare the biological activity of carbonic nano-structures of natural and artificial origination, namely, humic substances (HS) and fullerenols. Materials and methods: The representative of the fullerenol group, С 60 О y (OH) x where у + x = 20–22, was chosen. Enzyme-based luminescent bioassay was applied to evaluate toxicity and antioxidant properties of HS and fullerenol (F); chemiluminescent luminol method was used to study a content of reactive oxygen species (ROS) in the solutions. Toxicity of the bioactive compounds was evaluated using effective concentrations ЕС 50 ; detoxification coefficients D OxT were applied to study and compare antioxidant activity of the compounds. Antioxidant activity and ranges of active concentrations of the bioactive compounds were determined in model solutions of organic and inorganic oxidizers—1,4-benzoquinone and potassium ferricianide. Results and discussion: Values of ЕС 50 revealed higher toxicity of HS than F (0.005 and 0.108 g L −1 , respectively); detoxifying concentrations of F were found to be lower. Antioxidant ability of HS was demonstrated to be time-dependent; the 50-min preliminary incubation in oxidizer solutions was suggested as optimal for the detoxification procedure. On the contrary, F’ antioxidant effect demonstrated independency on time. Antioxidant effect of HS did not depend on amphiphilic characteristics of the media (values of D OxT were 1.3 in the solutions of organic and inorganic oxidizers), while this of F was found to depend: it was maximal (D OxT = 2.0) in solutions of organic oxidizer, 1,4-benzoquinone. Conclusions: Both HS and F demonstrated toxicity and low-concentration antioxidant ability; however, quantitative characteristics of their effects were different. The differences were explained with HS polyfunctionality, higher ability to decrease ROS content, non-rigidity, and diffusion restrictions in their solutions. Antioxidant effect of the bioactive compounds was presumably attributed to catalytic redox activity of their π-fragments. The paper demonstrates a high potential of luminescent enzymatic bioassay to study biological activity of nano-structures of natural and artificial origination.

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Журнал: Journal of Soils and Sediments

Номера страниц: 2689-2696

ISSN журнала: 14390108

Издатель: Ecomed Verlagsgesellschaft mbH


  • Sachkova A.S. (National Research Tomsk Polytechnic University)
  • Kovel E.S. (Institute of Physics FRC KSC SB RAS)
  • Kudryasheva N.S. (Siberian Federal University)
  • Churilov G.N. (Institute of Physics FRC KSC SB RAS)
  • Stom D.I. (Irkutsk National Research Technical University)

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