Тип публикации: статья из журнала
Год издания: 2020
Идентификатор DOI: 10.1016/j.ijbiomac.2020.07.095
Ключевые слова: cupriavidus necator b-10646, emulsifiers, fatty acids, plant oils, polyhydroxyalkanoates
Аннотация: The study addresses the growth of the wild-type strain Cupriavidus necator B-10646 and synthesis of polyhydroxyalkanoates by this strain on media containing plant oils with different compositions of fatty acids: palm, Siberian oilseed, and refined and unrefined sunflower seed oils. The study showed that the best carbon substrate waПоказать полностьюs palm oil. Comparison of fatty acid compositions of the starting oils and unutilized residual substrates showed that C. necator B-10646 cells consumed the fatty acids from palm oil evenly while in experiments with other oils, they utilized polyenoic fatty acids first. Higher production parameters of the culture were obtained by preparation of emulsified oil medium using Tween 80 and sodium cocoyl glutamate as emulsifiers. All polyhydroxyalkanoate specimens were terpolymers that contained 3-hydroxybutyrate as the major component and minor amounts of 3-hydroxyvalerate (0.9–1.9 mol%) and 3-hydroxyhexanoate (0.5–1.1 mol%). Molecular weight of polyhydroxyalkanoate specimens depended on the type of plant oil and emulsifier. © 2020
Журнал: International Journal of Biological Macromolecules
Выпуск журнала: Vol. 164
Номера страниц: 121-130
ISSN журнала: 01418130