Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1183/1393003.congress-2017.PA2749

Аннотация: Mycobacterium tuberculosis (Mtb) is an infectious agent that causes tuberculosis (TB) in humans. Studies of the mechanisms of Mtb survival and their replication in the hosts are extremely important for the development of new vaccines and methods for TB treatment because of the emergence and spread of high-virulence strains of Mtb. Показать полностьюPreviously, we have established the method for the production of ex vivo cultures of alveolar macrophages (Mph) from resected lungs of patients with pulmonary TB. We have observed the formation of irregular aggregates in colonies or, for the first time, cords (Fig. 1) by replicating Mtb in alveolar Mph of some TB patients. Cording is morphology of Mtb growth in colonies, in which Mtb are arranged in the form of plaits, bundles, or cords, while drawing up in a line along their long axes and in close parallel arrangement. As is known, the cord phenotype of Mtb growth on agar correlates with the virulence of Mtb. Here, our aim is to determine the correlation between Mtb cording in human alveolar Mph and virulence of Mtb in guinea pig TB model. In a result, we established the correlation between the formation of Mtb cords in human Mph and high Mtb virulence in guinea pigs. Thus, this marker may be used for quick estimation of Mtb virulence for TB patients.

Журнал: European Respiratory Journal, Supplement

Выпуск журнала: Т. 50, № S61

Номера страниц: 2749

ISSN журнала: 09041850

Издатель: European Respiratory Society

• Ufimtseva Elena (Research Institute of Biochemistry, Federal Research Center of Fundamental and Translational Medicine , Scientific Department)
• Eremeeva Natalya (Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation)
• Kravchenko Marionella (Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation)
• Vakhrusheva Diana (Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation)
• Skornyakov Sergey (Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation)

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