Тип публикации: статья из журнала

Год издания: 2019

Идентификатор DOI: 10.1016/j.tube.2018.12.002

Ключевые слова: mycobacterium tuberculosis, virulence, patients with pulmonary tuberculosis, alveolar macrophages, Phagosomes, guinea pig

Аннотация: Tuberculosis (TB) is a dangerous airborne disease caused byMycobacterium tuberculosis(Mtb) and characterizedby a tight interplay between pathogen and host cells, mainly alveolar macrophages. Studies of the mechanisms ofMtbsurvival within human cells during TB disease are extremely important for the development of new stra-tegies anПоказать полностьюd drugs for TB treatment. We have used theex vivocultures of alveolar macrophages and histologicalsections obtained from the resected lungs of patients with pulmonary TB to establish the unique features ofMtblifestyle in host cells. Our data indicate thatMtbwith different virulence, as single and in colonies, with orwithout cording morphology, are exclusively intravacuolar pathogens with intact phagosomal membranes inviable host cells of TB patients andMtb-infected guinea pig. Mycobacteria were detected in the cytoplasm and/ordamaged vacuoles only in alveolar macrophages with morphological signs of cell death after prolongedex vivoculture, howeverMtbwere found inside phagosomes in viable alveolar macrophages or cells with apoptotic/necrotic morphology in the sameex vivocell culture. TheMtbphagosomes interacted with human differentendocytic pathways, but inhibited phagolysosomal biogenesis, while intracellular vesicles containingMtbpro-ducts were fused with lysosomes in the same host cells.

Журнал: Tuberculosis

Выпуск журнала: Т. 114

Номера страниц: 77-90

ISSN журнала: 14729792

Издатель: Churchill Livingstone

• Ufimtseva E (Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health ofthe Russian Federation, 50 XXII Partsyezda Street, 620039, Yekaterinburg, Russia)
• Eremeeva N (Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health ofthe Russian Federation, 50 XXII Partsyezda Street, 620039, Yekaterinburg, Russia)
• Bayborodin S (Shared Center for Microscopic Analysis of Biological Objects, Federal Research Center Institute of Cytology and Genetics, 10 Lavrentyeva Prospect, 630090, Novosibirsk,Russia)
• Umpeleva T (Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health ofthe Russian Federation, 50 XXII Partsyezda Street, 620039, Yekaterinburg, Russia)
• Vakhrusheva D (Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health ofthe Russian Federation, 50 XXII Partsyezda Street, 620039, Yekaterinburg, Russia)
• Skornyakov S (Scientific Department, Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health ofthe Russian Federation, 50 XXII Partsyezda Street, 620039, Yekaterinburg, Russia)

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