Тип публикации: статья из журнала
Год издания: 2018
Идентификатор DOI: 10.1002/prot.25387
Ключевые слова: CASP12, Protein interfaces, rosetta, Structure prediction, symmetric assemblies
Аннотация: Many naturally occurring protein systems function primarily as symmetric assemblies. Prediction of the quaternary structure of these assemblies is an important biological problem. This article describes automated tools we have developed for predicting the structures of symmetric protein assemblies in the Robetta structure predictioПоказать полностьюn server. We assess the performance of this pipeline on a set of targets from the recent CASP12/CAPRI blind quaternary structure prediction experiment. Our approach successfully predicted 5 of 7 symmetric assemblies in this challenge, and was assessed as the best participating server group, and 1 of only 2 groups (human or server) with 2 predictions judged as high quality by the assessors. We also assess the method on a broader set of 22 natively symmetric CASP12 targets, where we show that oligomeric modeling can improve the accuracy of monomeric structure determination, particularly in highly intertwined oligomers.
Журнал: Proteins: Structure, Function and Bioinformatics
Выпуск журнала: Т. 86
Номера страниц: 283-291
ISSN журнала: 08873585
Издатель: Wiley-Liss Inc