Тип публикации: статья из журнала
Год издания: 2015
Идентификатор DOI: 10.3897/CompCytogen.v9i3.4636
Ключевые слова: COI, Genitalia morphology, Histone H1, Intraspecific variation, Introgression, ITS2, Lepidoptera, Leptidea, Molecular markers, Wolbachia, WSP, Leptidea amurensis, Pieridae
Аннотация: In southern West Siberia, as many as four Leptidea Billberg, 1820 species are present sympatrically: L. amurensis (Menetries, 1859), L. morsei (Menetries, 1859), L. sinapis (Linnaeus, 1758) and L. juvernica Williams, 1946. The two latter were recently recognised as nearly sibling species on morphological and molecular characters. SПоказать полностьюpecimens intermediate as to their subtle diagnostic characters occurring in West Siberia and elsewhere were interpreted as resulted from limited introgression. This supposition was tested via populational morphological and molecular analysis of spring brood specimens of all the four species taken from a limited (4.5 ? 0.2 km) area in the suburbs of Novosibirsk. The samples were analysed with respect to the genitalic morphology, external characters, three nuclear (CAD, H1 gene and ITS2) and one mitochondrial (COI) molecular markers, infection of the intracellular maternally inherited bacterial symbiont Wolbachia Hertig, 1836 and its wsp gene coding for a hypervariable surface protein. Interspecific variation of the nuclear CAD and ITS2 sequences and the mitochondrial COI gene in L. sinapis and L. juvernica turned out concordant. The absence of molecular evidence of introgression suggests genetic integrity of these two species and allows their reliable identification by molecular characters. The genitalic (lengths of the saccus and valva) and external characters (wing pattern) of males overlap in L. sinapis and L. juvernica, as identified by molecular markers and thus are not so helpful in actual species identification. Only the ductus bursae length showed no overlap and can be used for identification of females. The histone H1 gene appeared five times less variable over the four studied species than COI, and found to be identical in species L. sinapis and L. juvernica. Wolbachia infection was found in all studied species. We identified three wsp variants of Wolbachia: 1) wsp-10 allele in L. amurensis, L. sinapis, L. juvernica; 2) a very similar wsp-687 allele in L. sinapis; and 3) wsp-688, highly divergent to the previous ones, in L. morsei. © Vladimir I. Solovyev et al.
Журнал: Comparative Cytogenetics
Выпуск журнала: Vol. 9, Is. 3
Номера страниц: 299-324