Тип публикации: статья из журнала
Год издания: 2007
Идентификатор DOI: 10.1016/j.enzmictec.2006.05.003
Ключевые слова: bioluminescence; bacterial luciferase; NADH : FMN-oxidoreductase; immobilization; bioluminescent assays
Аннотация: A disk-shaped reagent based on bacterial NADH:FMN-oxidoreductase and luciferase immobilized into a starch gel is developed. The reagent characteristics, such as activity and stability depend on the time of drying, the initial luciferase preparation content, the source of the starch and its gel concentration. The experimental error Показать полностьюof bioluminescence determination does not exceed 10-15%. The method of immobilization into gel provides the coimmobilization luciferase and NADH:FMN-oxidoreductase and their substrates. On the basis of these reagents, the multifunctional immobilized biosensor for bioluminescent assay in medicine, ecology and other fields may be developed. (c) 2006 Elsevier Inc. All rights reserved.
Журнал: ENZYME AND MICROBIAL TECHNOLOGY
Выпуск журнала: Vol. 40, Is. 2
Номера страниц: 343-346
ISSN журнала: 01410229
Место издания: NEW YORK
Издатель: ELSEVIER SCIENCE INC