Тип публикации: статья из журнала
Год издания: 2015
Идентификатор DOI: 10.1007/s10517-015-3049-8
Ключевые слова: neutrophilic granulocytes, metabolism, enzyme activities, dehydrogenases, peritonitis
Аннотация: Bioluminescent method for measurements of the neutrophilic NAD(P)-dependent dehydrogenases (lactate dehydrogenase, NAD-dependent malate dehydrogenase, NADP-dependent decarboxylating malate dehydrogenase, NAD-dependent isocitrate dehydrogenase, and glucose-6-phosphate dehydrogenase) is developed. The sensitivity of the method allowsПоказать полностьюminimization ?of the volume of biological material for measurements to 10?4?neutrophils per analysis. The ?method is tried in patients with diffuse purulent peritonitis. Low levels of NADPH synthesis ?enzymes and high levels of enzymes determining the substrate flow by the Krebs cycle found ?in these patients can lead to attenuation of functional activity of cells.
Журнал: Bulletin of Experimental Biology and Medicine
Выпуск журнала: Т. 159, № 5
Номера страниц: 692-695
ISSN журнала: 00074888
Место издания: Москва
Издатель: Springer New York Consultants Bureau