Towards biological quantity theory for nominal property metrology in polyenzymatic devices with living cells

Описание

Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций

Конференция: Joint IMEKO TC1-TC7-TC13-TC18 Symposium; St Petersburg, RUSSIA; St Petersburg, RUSSIA

Год издания: 2019

Идентификатор DOI: 10.1088/1742-6596/1379/1/012036

Аннотация: Here we discuss the concepts of "biological quantity" and "nominal property" within the framework of the problem of biological measurements based on new specific results of biological analysis using a microfluidic platform and chips developed by our team earlier. It was shown that based on different microfluidic platforms it is posПоказать полностьюsible to develop chips with a polyenzymatic bioluminescent system NAD(P)H:FMN-oxidoreductase-luciferase (Red + Luc), which can be used in various areas of biological analysis. Thus, disposable microfluidic chips with Red + Luc system suitable for field and indoor use were developed using continuous flow microfluidic platform. The use of droplet-based microfluidic platform allowed to develop microfluidic chips with Red + Luc system for long-term continuous measurements of water samples, for example, in places of waste disposal by industrial enterprises. The reference for comparing different biological quantities with each other in the proposed system was a photodetector, which converted non-numeric values and nominal properties recorded by a biological module Red + Luc into numerical variables. Such a reference was implemented as a portable luminometer based on silicon photomultiplier. The results allow to perform other biological measurements and to start the discussion of modern biological concepts in the language of biological measures. © 2019 IOP Publishing Ltd. All rights reserved.

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Издание

Журнал: Journal of Physics: Conference Series

Выпуск журнала: Vol. 1379, Is. 1

Номера страниц: 12036

ISSN журнала: 17426588

Издатель: Institute of Physics Publishing

Авторы

  • Belobrov P.I (Siberian Fed Univ, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia)
  • Evstrapov A.A. (RAS, Inst Analyt Instrumentat, St Petersburg 190103, Russia)
  • Esimbekova E.N. (Siberian Fed Univ, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia; SB RAS, Lab Photobiol, Inst Biophys, Krasnoyarsk 660036, Russia)
  • Denisov I.A. (Siberian Fed Univ, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia; SB RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Lab Digital Controlled Drugs & Theranost, Krasnoyarsk 660036, Russia)
  • Lukyanenko K.A. (Siberian Fed Univ, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia; SB RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, Lab Digital Controlled Drugs & Theranost, Krasnoyarsk 660036, Russia)
  • Osipova E.D. (Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia)
  • Yakimov A.S. (Krasnoyarsk State Med Univ, Res Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia)

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