Тип публикации: статья из журнала
Год издания: 2013
Идентификатор DOI: 10.1016/j.biortech.2013.07.070
Ключевые слова: Cupriavidus eutrophus, Polyhydroxyalkanoates, Autotrophic synthesis, 3-Hydroxyhexanoate, Hydrogen-oxidizing bacteria, Nitrogen deficiency, Oxygen mass transfer, Physicomechanical properties, Poly-3-hydroxybutyrate, Nitrogen, Substrates, Carbon dioxide, 3 hydroxybutyric acid, 4 hydroxybutyric acid, gamma butyrolactone, hexanoic acid, hydrogen, manganese, phosphorus, poly(3 hydroxybutyric acid), polyhydroxyalkanoic acid, potassium, sulfur, valeric acid, autotrophy, bacterium, cell organelle, chemostat, mass transfer, physicochemical property, polymer, substrate, article, bacterial growth, bacterium culture, cell density, cell growth, concentration (parameters), Cupriavidus, flow rate, nonhuman, precursor, priority journal, synthesis, 3-Hydroxybutyric Acid, Biodegradation, Environmental, Bioreactors, Carbon, Clostridium beijerinckii, Crystallization, Fermentation, Gases, Oxygen, Polymers, Refuse Disposal, Temperature
Аннотация: Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D = 0.1 h(-1), on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limitinПоказать полностьюg elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460 h(-1), P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50 g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, gamma-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties. (c) 2013 Elsevier Ltd. All rights reserved.
Журнал: BIORESOURCE TECHNOLOGY
Выпуск журнала: Vol. 146
Номера страниц: 215-222
ISSN журнала: 09608524
Место издания: OXFORD
Издатель: ELSEVIER SCI LTD