Тип публикации: статья из журнала

Год издания: 2022

Идентификатор DOI: 10.1016/j.bbrc.2022.02.011

Ключевые слова: competitive assay, genetic fusion, photoprotein obelin, tumor marker

Аннотация: Two variants of Ca2+-regulated photoprotein obelin, extended from the N-terminus with small tumor markers – melanoma inhibitory activity protein (MIA) and survivin, one of the protein inhibitors of apoptosis, were designed, obtained and studied. Both domains in the obtained hybrid proteins exhibit the properties of the initial moleПоказать полностьюcules: the main features of Ca2+-triggered bioluminescence are close to those of obelin, and the tumor markers’ domains are recognized and bound by the corresponding antibodies. The obtained hybrids compete with the corresponding tumor markers for binding with antibodies, immobilized on the surface and their use has been shown to be promising as bioluminescent labels in a one-stage solid-phase competitive immunoassay. © 2022

Журнал: Biochemical and Biophysical Research Communications

Выпуск журнала: Vol. 598

Номера страниц: 69-73

ISSN журнала: 0006291X

Издатель: Elsevier B.V.

• Bashmakova E.E. (Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation)
• Panamarev N.S. (Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)
• Kudryavtsev A.N. (Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation)
• Frank L.A. (Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation)

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