Biodegradation of polyhydroxyalkanoates (PHAs) in the South China Sea and identification of PHA-degrading bacteria


Тип публикации: статья из журнала

Год издания: 2011

Идентификатор DOI: 10.1134/S0026261711020184

Ключевые слова: polyhydroxyalkanoates, PHA, biodegradation in marine environments, microbial PHA degraders, Bacillus (bacterium), Bacillus sp., Bacteria (microorganisms), Enterobacter, Enterobacter cloacae, Gracilibacillus, Prospect Hill virus

Аннотация: The biodegradation patterns of two types of PHA, a 3-hydroxybutyrate (3-PHB) polymer and a 3-hydroxybutyrate and 3-hydroxyvalerate (3-PHB/3-PHV) copolymer, were studied in tropical marine environments (Dam Bay, South China Sea, Nha Trang, Vietnam). No reliable differences in the degradation of 3-PHB and 3-PHB/3-PHV were revealed. IПоказать полностьюt was shown that the degradation process depended mainly on the shape of a polymer product and its production method: the degradation of polymer films was found to be more active than that of molded solids. A decrease in the molecular mass of both types of PHA was detected in the course of the degradation of PHA samples. However, the degree of PHA crystallinity did not change; that is, the levels of degradation of both the amorphous and crystalline phases of PHA were almost the same. Among microbial PHA degraders, three bacterial strains, Bacillus sp. IBP-V002, Enterobacter cloacae sp. IBP-V001, and Gracilibacillus sp. IBP-V003, were identified based on the results of morphological, biochemical, and molecular phylogenetic analyses. The ability of the representatives of the genera Gracilibacillus and Enterobacter to degrade PHA was revealed for the first time.

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Выпуск журнала: Vol. 80, Is. 2

Номера страниц: 252-260

ISSN журнала: 00262617

Место издания: NEW YORK



  • Volova T.G. (Siberian Federal University)
  • Dung V.V. (Marine Department,Joint Russian-Vietnamese Tropical Research and Test Center)
  • Boyandin A.N. (Siberian Federal University)
  • Gitel'zon I.I. (Institute of Biophysics, Siberian Branch,Russian Academy of Sciences)
  • Kozhevnikov I.V. (Siberian Federal University)
  • Prudnikova S.V. (Siberian Federal University)
  • Vasil'ev A.D. (Kirensky Institute of Physics, Siberian Branch,Russian Academy of Sciences)
  • Karpov V.A. (Severtsov Institute of Ecology and Evolution,Russian Academy of Sciences)
  • Rudnev V.P. (Marine Department,Joint Russian-Vietnamese Tropical Research and Test Center)
  • Xuan B.B. (Marine Department,Joint Russian-Vietnamese Tropical Research and Test Center)