Тип публикации: статья из журнала

Год издания: 2000

Ключевые слова: intein, Protein ligation, RabGGTase, Rab escort protein, Rab7

Аннотация: Rab GTPases play a key role in the regulation of membrane traffic. Posttranslational geranylgeranylation is critical for their biological activity and is conferred by a Rab geranylgeranyl transferase (RabGGTase). To study the interactions between Rab proteins and RabGGTase, we used in vitro ligation methodology to generate a fluoreПоказать полностьюscent semi-synthetic Rab7 protein. The obtained protein was functionally active and was used to demonstrate a micromolar affinity interaction of Rab7 with the RabGGTase in the absence of Rab escort protein (REP). This finding is consistent with an earlier proposed model according to which RabGGTase possesses two independent weak binding sites for REP and Rab proteins.

Журнал: FEBS Letters

Выпуск журнала: Т. 468, № 2-3

Номера страниц: 155-158

ISSN журнала: 00145793

Издатель: Elsevier Science Publishing Company, Inc.

• Iakovenko A.
• Rostkova E.
• Merzlyak E.
• Hillebrand A.M.
• Thoma N.H.
• Goody R.S.
• Alexandrov K. (K. Alexandrov, Department of Physical Biochemistry, Max-Planck Institute for Molecular Physiology, Otto-Hahn-Strasse 11, 44227, Dortmund, Germany)

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