Тип публикации: статья из журнала
Год издания: 2008
Идентификатор DOI: 10.1070/QE2008v038n07ABEH013558
Ключевые слова: carboxyhemoglobin, photodissociation, natural photolysis conditions, Animal cell culture, Dissociation, Irradiation, Photolysis, Porphyrins, Solutions, At wavelengths, Average powers, Buffer solutions, Experimental conditions, Flash photolysises, Human bloods, Irradiation intensities, Model experiments, Photoinduced dissociations, Q transitions, Radiation wavelengths, Saturation degrees, Set-ups
Аннотация: The general properties of photodissociation of carboxyhemoglobin (HbCO) in buffer solutions of whole human blood are studied by the flash photolysis method on a setup with intersecting beams. It is shown that the efficiency of photoinduced dissociation of the HbCO complex virtually linearly depends on the photolytic irradiation intПоказать полностьюensity for the average power density not exceeding 45 mW cm(-2). The general dissociation of the HbCO complex in native conditions occurs in a narrower range of values of the saturation degree than in model experiments with the hemoglobin solution. The dependence of the pulse photolysis efficiency of HbCO on the photolytic radiation wavelength in the range from 550 to 585 nm has a broad bell shape. The efficiency maximum corresponds to the electronic Q transition (porphyrin pi - pi* absorption) in HbCO at a wavelength of 570 nm. No dissociation of the complex was observed under given experimental conditions upon irradiation of solutions by photolytic radiation at wavelengths above 585 nm.
Журнал: QUANTUM ELECTRONICS
Выпуск журнала: Vol. 38, Is. 7
Номера страниц: 695-701
ISSN журнала: 10637818
Место издания: LETCHWORTH
Издатель: TURPION LTD