Тип публикации: статья из журнала

Год издания: 2002

Идентификатор DOI: 10.1007/s00424-002-0789-x

Ключевые слова: luciferase assay, NaSi-1, responsive element, Sulfate homeostasis, sulfate transporter, transcriptional regulation, triiodothyronine, vitamin D

Аннотация: The renal sodium-sulfate cotransporter, NaS_i-1, a protein implicated to control serum sulfate levels, has been shown to be regulated in vivo by 1,25-dihydroxyvitamin D₃ (1,25-(OH)₂D₃) and tri-iodothyronine (T₃). Recently, we cloned the mouse NaS_i-1 gene (Nas1) and in the present study identified a 1,25-(OH)₂D₃- and T₃-responsive element located within the Nas1 promoter. Mutational analysis of the Nas1 promoter resulted in identification of a direct repeat 6-type vitamin-D-responsive element (DR6 VDRE) at -525 to -508 and an imperfect inverted repeat 0-type T₃-responsive element (IR0 T₃RE) at -436 to -425 which conferred 1,25-(OH)₂D₃ and T₃ responsiveness, respectively. In summary, we have identified responsive elements that mediate the enhanced transcription of Nas1 by 1,25-(OH)₂D₃ and T₃, and these mechanisms may provide important clues to the physiological control of sulfate homeostasis.

Журнал: Pflügers Archiv: European Journal of Physiology

Выпуск журнала: Т. 444, № 3

Номера страниц: 353-359

ISSN журнала: 00316768

Издатель: Springer-Verlag GmbH

• Dawson P.A. (School of Biomedical Sciences, Department of Physiology and Pharmacology, University of Queensland, Brisbane 4072, St. Lucia, QLD 4072, Australia)
• Markovich D. (School of Biomedical Sciences, Department of Physiology and Pharmacology, University of Queensland, Brisbane 4072, St. Lucia, QLD 4072, Australia)

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