Тип публикации: статья из журнала
Год издания: 2013
Идентификатор DOI: 10.1039/c2ay25976a
Ключевые слова: Gene encoding, Allelic variants, Analytes, Binding assays, Factor V Leiden, Genotyping, Good correlations, High-throughput, Number of samples, Simultaneous detection, Single nucleotide polymorphisms, Single well, Time resolution, Traditional techniques, Bioluminescence
Аннотация: Two obelin mutants distinctly different in the color of bioluminescence were successfully applied to simultaneous detection of two analytes in a single well. The flash-type signals were triggered by single injection of Ca2+ and were discriminated by the spectral and time resolutions. The technique was developed in a high-throughputПоказать полностьюformat, and applied to simultaneous immunoassay of two gonadotropic hormones - luteinizing (lutropin or hLH) and follicle stimulating (hFSH) - in clinical sera, and to simultaneous detection of two gene allelic variants at single nucleotide polymorphism genotyping of the human F5 gene encoding factor V Leiden polymorphism 1691 G -> A (R506Q). A considerable number of samples were investigated and the results obtained were in good correlation with those obtained by using traditional techniques.
Журнал: ANALYTICAL METHODS
Выпуск журнала: Vol. 5, Is. 3
Номера страниц: 636-640
ISSN журнала: 17599660
Место издания: CAMBRIDGE
Издатель: ROYAL SOC CHEMISTRY