Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций
Год издания: 2014
Идентификатор DOI: 10.1007/978-3-662-43385-0_6
Ключевые слова: Bioluminescence, Ca2+-regulated photoprotein, Diagnostics, Immunoassay, Luciferase, Nucleic acid hybridization assay, enzyme, animal, diagnosis, human, luminescence, metabolism, nucleic acid hybridization, procedures, Animals, Enzymes, Humans, Luminescent Measurements
Аннотация: Nowadays luciferases are effectively used as analytical instruments in a great variety of research fields. Of special interest are the studies dealing with elaboration of novel analytical systems for the purposes of medical diagnostics. The ever-expanding spectrum of clinically important analytes accounts for the increasing demand Показать полностьюfor new techniques for their detection. In this chapter we have made an attempt to summarize the results on applications of luciferases as reporters in binding assays including immunoassay, nucleic acid hybridization assay, and so on. The data over the last 15 years have been analyzed and clearly show that luciferase-based assays, due to extremely high sensitivity, low cost, and the lack of need for skilled personnel, hold much promise for clinical diagnostics.
Журнал: BIOLUMINESCENCE: FUNDAMENTALS AND APPLICATIONS IN BIOTECHNOLOGY, VOL 1
Выпуск журнала: Vol. 144
Номера страниц: 175-197
ISSN журнала: 07246145
Место издания: BERLIN
Издатель: SPRINGER-VERLAG BERLIN