Тип публикации: статья из журнала

Год издания: 2014

Идентификатор DOI: 10.1134/S1062360414020064

Ключевые слова: Pinus sibirica, megagametophytes, somatic embryogenesis, embryos

Аннотация: Megagametophytes of Siberian pine were cultured on an in vitro culture medium 1/2 LV supplemented with growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (6-BAP) to form embryos. The competency of somatic cell of explants to embryogenesis manifested itself in an organized growth and polarity. A coenocyteПоказать полностьюconsisting of long vacuolated cells was formed in the megagametophyte culture. Then, the migration of the nuclei to one of the poles of the cell, their division, and formation of embryoids was observed. The megagametophyte culture of the Siberian pine differed from the zygotic embryo culture by the absence of asymmetric division in the vacuolated cell.

Журнал: RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY

Выпуск журнала: Vol. 45, Is. 2

Номера страниц: 93-100

ISSN журнала: 10623604

Место издания: NEW YORK

Издатель: MAIK NAUKA/INTERPERIODICA/SPRINGER

• Tret'yakova I.N. (Sukachev Institute of Forestry, Siberian Branch, Russian Academy of Sciences)
• Voroshilova E.V. (Siberian Federal University)

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