Development and characterization of novel 2′-F-RNA aptamers specific to human total and glycated hemoglobins

Описание

Тип публикации: статья из журнала

Год издания: 2019

Идентификатор DOI: 10.1016/j.ab.2019.02.004

Ключевые слова: guanosine, 118-00-3, hemoglobin A1c, 62572-11-6

Аннотация: Aptamers are short DNA and RNA fragments which bind their molecular targets with affinity and specificity comparable to those of antibodies. Here, we describe the selection of novel 2′-F-RNA aptamers against total human hemoglobin or its glycated form HbA1c. After SELEX and high-throughput sequencing of the enriched libraries, affiПоказать полностьюnities and specificities of candidate aptamers and their truncated variants were examined by the solid-phase bioluminescent assay. As a result, we identified aptamers specific to both hemoglobins or only glycated HbA1c. The developed 2′-F-RNA aptamers have shown their applicability for detection of total and glycated hemoglobin in one sample. © 2019 Elsevier Inc. Aptamers are short DNA and RNA fragments which bind their molecular targets with affinity and specificity comparable to those of antibodies. Here, we describe the selection of novel 2?-F-RNA aptamers against total human hemoglobin or its glycated form HbA

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Издание

Журнал: Analytical Biochemistry

Выпуск журнала: Vol. 570

Номера страниц: 43-50

ISSN журнала: 00032697

Издатель: Academic Press Inc.

Авторы

  • Davydova Anna (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)
  • Vorobyeva Mariya (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)
  • Bashmakova Eugenia (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia; Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk, Russia)
  • Vorobjev Pavel (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia; Novosibirsk State Univ, Dept Nat Sci, Pirogova St 2, Novosibirsk 630090, Russia)
  • Krasheninina Olga (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)
  • Tupikin Alexey (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)
  • Kabilov Marsel (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)
  • Krasitskaya Vasilisa (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia; Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk, Russia)
  • Frank Ludmila (Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk, Russia; Siberian Fed Univ, Krasnoyarsk 660041, Russia)
  • Venyaminova Alya (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)

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