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Development and characterization of novel 2′-F-RNA aptamers specific to human total and glycated hemoglobins

Описание

Тип публикации: статья из журнала

Год издания: 2019

Идентификатор DOI: 10.1016/j.ab.2019.02.004

Ключевые слова: guanosine, 118-00-3, hemoglobin A1c, 62572-11-6

Аннотация: Aptamers are short DNA and RNA fragments which bind their molecular targets with affinity and specificity comparable to those of antibodies. Here, we describe the selection of novel 2′-F-RNA aptamers against total human hemoglobin or its glycated form HbA1c. After SELEX and high-throughput sequencing of the enriched libraries, affiПоказать полностьюnities and specificities of candidate aptamers and their truncated variants were examined by the solid-phase bioluminescent assay. As a result, we identified aptamers specific to both hemoglobins or only glycated HbA1c. The developed 2′-F-RNA aptamers have shown their applicability for detection of total and glycated hemoglobin in one sample. © 2019 Elsevier Inc.

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Издание

Журнал: Analytical Biochemistry

Выпуск журнала: Vol. 570

Номера страниц: 43-50

ISSN журнала: 00032697

Издатель: Academic Press Inc.

Персоны

  • Davydova Anna (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)
  • Vorobyeva Mariya (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)
  • Bashmakova Eugenia (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia; Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk, Russia)
  • Vorobjev Pavel (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia; Novosibirsk State Univ, Dept Nat Sci, Pirogova St 2, Novosibirsk 630090, Russia)
  • Krasheninina Olga (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)
  • Tupikin Alexey (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)
  • Kabilov Marsel (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)
  • Krasitskaya Vasilisa (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia; Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk, Russia)
  • Frank Ludmila (Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk, Russia; Siberian Fed Univ, Krasnoyarsk 660041, Russia)
  • Venyaminova Alya (Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia)

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