Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1039/c7ay00535k

Ключевые слова: Proteins, Recombinant proteins, Viruses, Binding proteins, Bioluminescence

Аннотация: A one-step bioluminescent immunoassay for tick-borne encephalitis virus (TBEV) in natural ticks based on the hybrid protein 14D5a-Rm7 was developed. Recombinant Ca2+-dependent coelenterazine-binding protein was shown to be a more convenient substrate form for the Rm7 domain than coelenterazine. Over 600 samples of natural ticks werПоказать полностьюe analyzed and shown to have essential differences in the discrimination factor D for TBEV-positive (2.77 ± 0.81) and TBEV-negative (1.15 ± 0.28) samples. © The Royal Society of Chemistry.

Журнал: Analytical Methods

Выпуск журнала: Vol. 9, Is. 15

Номера страниц: 2252-2255

• Kudryavtsev A.N. (Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia)
• Burakova L.P. (Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia)
• Frank L.A. (Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Krasnoyarsk 660041, Russia)

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