Calcium signaling in tumor cells


Тип публикации: статья из журнала

Год издания: 2016

Ключевые слова: Ehrlich ascite adenocarcinoma, Ca2+, Ca-ATPase, membrane microviscosity, apoptosis, necrosis

Аннотация: The objective. The objective of this study was to investigate change in calcium maintenance of Ehrlich ascite adenocarcinoma cells in the course of tumor growth. Materials and methods. Cell viability was determined by counting apoptotic and necrotic cells under the fluorescence microscope. Intracellular calcium concentration was deПоказать полностьюtermined with the fluorescent probe 2,5 mM Fura-2AM. Relative microviscosity of membranes was estimated by fluorescence method using Pyrene. The activity of Ca-ATPase was assessed by the amount of inorganic phosphate produced during ATP hydrolysis. Results and discussions. We revealed that dynamics of Ehrlich ascite adenocarcinoma growth in our experiments was different from the commonly observed one. We noticed one peculiarity. The quantity of cells decreased on the day 12 after the tumor transplantation. This diminution was caused by mitotic activity lowering and by increase of apoptotic cell quantity the day before. Ehrlich ascite adenocarcinoma growth was accompanied by change in intracellular calcium maintenance which was independent of Ca-ATPase and cell membrane microviscosity. Conclusions. Thus, our experiments have shown the change in calcium concentration. Apparently Ca2+ activated proliferation. However calcium maintenance was settled by ROS. Possibly ROS supression in tumour on the day 12 was able to put down proliferative processes stimulation.

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Журнал: Сибирское медицинское обозрение

Выпуск журнала: 3

Номера страниц: 27-33

ISSN журнала: 18199496

Место издания: Красноярск

Издатель: Государственное бюджетное образовательное учреждение высшего профессионального образования Красноярский государственный медицинский университет имени профессора В.Ф.Войно-Ясенецкого Министерства здравоохранения Российской Федерации


  • Zamay Anna S. (Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University)
  • Kolovskaya Olga S. (Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University)
  • Titova Nadegda M. (Siberian Federal University)
  • Chesnokova Larisa L. (Voino-Yasenetskii State Medical University, Ministry of Health of the Russian Federation)
  • Maltceva Elena A. (Voino-Yasenetskii State Medical University, Ministry of Health of the Russian Federation)
  • Zamay Tatiana N. (Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University)

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