Тип публикации: статья из журнала
Год издания: 2011
Идентификатор DOI: 10.1002/bio.1267
Ключевые слова: bioluminescence, enclosed environmental chambers, luciferase, pyridine nucleotides, stress in plants, nicotinamide adenine dinucleotide phosphate, article, luminescence, metabolism, physiological stress, physiology, Raphanus, NADP, Stress, Physiological
Аннотация: The application of bioluminescent sensors for monitoring key metabolites and enzymes that are indicators of stress in plants is demonstrated. The sensitivity of bioluminescent assay for NAD(P)H and NAD(P)(+) was about 0.5 and 1 nmol, respectively. The levels of NAD(P)H and NAD(P)(+) in radish (Raphanus sativus) root extracts from cПоказать полностьюontrols and from stress-induced conditions were compared. To induce environmental stress, the plants were grown in enclosed environmental chambers with low pressure (9 or 32 kPa), high humidity (>80%) and low oxygen partial pressure (down to 3.3-6.5 kPa). The concentrations of NAD(P)(+) and NAD(P)H in plants varied under stress conditions. Decreasing both total pressure from 101.5 to 32 or 9 kPa and partial pressure of oxygen increased the ratio of NAD(P)(+)/NAD(P)H from 0.2 to 4 or 6, respectively. The increase in this ratio suggests that plants are undergoing stress in these hypobaric environments. The developed bioluminescent assay for quantification of pyridine nucleotides in plant tissues is rapid, low-cost and easily performed. Copyright (C) 2011 John Wiley & Sons, Ltd.
Журнал: LUMINESCENCE
Выпуск журнала: Vol. 26, Is. 6
Номера страниц: 543-546
ISSN журнала: 15227235
Место издания: MALDEN
Издатель: WILEY-BLACKWELL