Bioluminescent enzyme assay for the indication of plant stress in enclosed life support systems


Тип публикации: статья из журнала

Год издания: 2011

Идентификатор DOI: 10.1002/bio.1267

Ключевые слова: bioluminescence, enclosed environmental chambers, luciferase, pyridine nucleotides, stress in plants, nicotinamide adenine dinucleotide phosphate, article, luminescence, metabolism, physiological stress, physiology, Raphanus, NADP, Stress, Physiological

Аннотация: The application of bioluminescent sensors for monitoring key metabolites and enzymes that are indicators of stress in plants is demonstrated. The sensitivity of bioluminescent assay for NAD(P)H and NAD(P)(+) was about 0.5 and 1 nmol, respectively. The levels of NAD(P)H and NAD(P)(+) in radish (Raphanus sativus) root extracts from cПоказать полностьюontrols and from stress-induced conditions were compared. To induce environmental stress, the plants were grown in enclosed environmental chambers with low pressure (9 or 32 kPa), high humidity (>80%) and low oxygen partial pressure (down to 3.3-6.5 kPa). The concentrations of NAD(P)(+) and NAD(P)H in plants varied under stress conditions. Decreasing both total pressure from 101.5 to 32 or 9 kPa and partial pressure of oxygen increased the ratio of NAD(P)(+)/NAD(P)H from 0.2 to 4 or 6, respectively. The increase in this ratio suggests that plants are undergoing stress in these hypobaric environments. The developed bioluminescent assay for quantification of pyridine nucleotides in plant tissues is rapid, low-cost and easily performed. Copyright (C) 2011 John Wiley & Sons, Ltd.

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Выпуск журнала: Vol. 26, Is. 6

Номера страниц: 543-546

ISSN журнала: 15227235

Место издания: MALDEN



  • Kratasyuk V. (Institute of Biophysics,SB RAS)
  • Esimbekova E. (Institute of Biophysics,SB RAS)
  • Correll M. (Agricultural and Biological Engineering Department,University of Florida)
  • Bucklin R. (Agricultural and Biological Engineering Department,University of Florida)

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