Morphological properties and levels of extracellular peroxidase activity and light emission of the basidiomycete Armillaria borealis treated with β-glucosidase and chitinase

Описание

Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.5943/mycosphere/8/4/11

Ключевые слова: basidiomycetes, bioluminescence, cell wall, β-Glucosidase, chitinase, peroxidase

Аннотация: The study estimates morphological properties and levels of extracellular peroxidase activity?and light emission of mycelium of the basidiomycete Armillaria borealis IBSO 2328 treated with?β-glucosidase and chitinase. Mycelium incubated with the enzymes shows considerable?morphological changes and indications of osmotic shock. InjurПоказать полностьюies observed in the cell envelope of?the fungal hyphae are primarily attributed to the partial (in the β-glucosidase treatment) or complete?(in the chitinase treatment) disintegration of the melanin layer on the surface of the cell wall.?Changes in the cell wall of hyphae are accompanied by release of extracellular peroxidases of the?fungus into the incubation medium and an increase in light emission relative to the luminescence of?the control pellets. We assume that higher level of luminescence of the enzyme-treated mycelium?samples could be related to the disintegration of the surface pigment layer of the hyphae and the?partial loss of extracellular peroxidases. The data obtained confirm the previously proposed?hypothesis in which light producing reaction of the fungus may be an additional way to neutralize?active oxygen radicals under stress.

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Издание

Журнал: Mycosphere

Выпуск журнала: Т. 8, 4

Номера страниц: 649-659

ISSN журнала: 20777019

Авторы

  • Mogilnaya O.A. (Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 660036 Krasnoyarsk, Russia)
  • Ronzhin N.O. (Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 660036 Krasnoyarsk, Russia)
  • Artemenko K.S. (Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 660036 Krasnoyarsk, Russia)
  • Bondar V.S. (Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 660036 Krasnoyarsk, Russia)

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