Тип публикации: статья из журнала
Год издания: 2020
Идентификатор DOI: 10.1007/s10924-020-01924-3
Ключевые слова: degradable p(3hb), various carbon substrates, films, structure, properties, nih 3t3 fibroblasts
Аннотация: Films were prepared from 2% solutions of biodegradable poly-3-hydroxybutyrate [P(3HB)] and investigated. The polymer was synthesized by theCupriavidus necatorB-10646 bacterium cultivated using various carbon sources (glucose and glycerol of different degrees of purity, containing 0.3 to 17.93% impurities). Glycerol as the substrateПоказать полностьюinfluenced molecular-weight properties and crystallinity of the polymer without affecting its temperature characteristics. The P(3HB) specimens synthesized from glycerol had reduced M-w(300-400 kDa) and degree of crystallinity (50-55%) compared to the specimens synthesized from glucose (860 kDa and 76%, respectively). The low-crystallinity P(3HB) specimens, regardless of the degree of purity of glycerol, produced a beneficial effect on the properties of polymer films, which had a better developed folded surface and increased hydrophilicity. The values of the highest roughness (R-a) of the films synthesized from glycerol were 1.8 to 4.0 times lower and the water angles 1.4-1.6 times smaller compared to the films synthesized from glucose (71.75 nm and 87.4 degrees, respectively). Those films performed better as cell scaffolds: the number of viable NIH fibroblasts was 1.7-1.9 times higher than on polystyrene (control) or films of P(3HB) synthesized from glucose.
Журнал: JOURNAL OF POLYMERS AND THE ENVIRONMENT
Выпуск журнала: Vol. 29, Is. 3
ISSN журнала: 15662543
Место издания: NEW YORK
Издатель: SPRINGER