Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes

Описание

Тип публикации: статья из журнала

Год издания: 1990

Идентификатор DOI: 10.1016/0378-1119(90)90117-A

Ключевые слова: Bioluminescence, expression in E. coli, luciferase, molecular evolution, nucleotide sequence, protein alignment, recombinant DNA, amino acid sequence, article, fungus, gene structure, genetic engineering, heredity, nonhuman, priority journal, vibrionaceae, Acyltransferases, Bacterial Proteins, Base Sequence, Cloning, Molecular, DNA, Bacterial, Genes, Bacterial, Luciferases, Luminescent Measurements, Molecular Sequence Data, Operon, Photobacterium, Restriction Mapping, Escherichia coli, Fungi, Photobacterium leiognathi, Vibrio harveyi

Аннотация: Genes encoding luminescence of Photobacterium leiognathi have been cloned in Escherichia coli. The luminescent clones were readily apparent. Among them, a clone containing a recombinant plasmid with a 13.5-kb insertion was identified. This DNA fragment contained all of the luminescence-encoding genes. The luciferase-encoding genes Показать полностью(lux) in this DNA fragment were localized. We have sequenced a part of the cloned lux region and identified the luxA, luxB and luxG genes encoding the ? and ? subunits of luciferase and a ? protein with an Mr of 26 180, respectively. The analysis of deduced amino acid sequences and comparison with known luciferase sequences from Vibrio harveyi, indicate the common origin of these proteins. © 1990.

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Издание

Журнал: Gene

Выпуск журнала: Vol. 86, Is. 1

Номера страниц: 89-94

Авторы

  • Illarrionov B.A.
  • Blinov V.M.
  • Douchenko A.P.
  • Protopopova M.V.
  • Karginov V.A.
  • Mertvetsov N.P.
  • Gitelson J.I.

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