From east to west across the Palearctic: Phylogeography of the invasive lime leaf miner Phyllonorycter issikii (Lepidoptera: Gracillariidae) and discovery of a putative new cryptic species in East Asia

Описание

Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1371/journal.pone.0171104

Аннотация: Knowing the phylogeographic structure of invasive species is important for understanding the underlying processes of invasion. The micromoth Phyllonorycter issikii, whose larvae damage leaves of lime trees Tilia spp., was only known from East Asia. In the last three decades, it has been recorded in most of Europe, Western Russia anПоказать полностьюd Siberia. We used the mitochondrial cytochrome c oxidase subunit I (COI) gene region to compare the genetic variability of P. issikii populations between these different regions. Additionally, we sequenced two nuclear genes (28S rRNA and Histone 3) and run morphometric analysis of male genitalia to probe for the existence of cryptic species. The analysis of COI data of 377 insect specimens collected in 16 countries across the Palearctic revealed the presence of two different lineages: P. issikiiand a putative new cryptic Phyllonorycterspecies distributed in the Russian Far East and Japan. In P. issikii, we identified 31 haplotypes among which 23 were detected in the invaded area (Europe) and 10 were found in its putative native range in East Asia (Russian Far East, Japan, South Korea and China), with only two common haplotypes. The high number of haplotypes found in the invaded area suggest a possible scenario of multiple introductions. One haplotype H1 was dominant (119 individuals, 67.2%), not only throughout its expanding range in Europe and Siberia but, intriguingly, also in 96% of individuals originating from Japan. We detected eight unique haplotypes of P. issikii in East Asia. Five of them were exclusively found in the Russian Far East representing 95% of individuals from that area. The putative new cryptic Phyllonorycterspecies showed differences from P. issikii for the three studied genes. However, both species are morphologically undistinguishable. They occur in sympatry on the same host plants in Japan (Sendai) and the Russian Far East (Primorsky krai) without evidence of admixture.

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Издание

Журнал: PLOS ONE

Выпуск журнала: Vol. 12, Is. 2

Номера страниц: 0171104

ISSN журнала: 19326203

Место издания: SAN FRANCISCO

Издатель: PUBLIC LIBRARY SCIENCE

Персоны

  • Kirichenko Natalia (Siberian Fed Univ, Krasnoyarsk, Russia;; ; INRA, Zool Forestiere UR0633, Orleans, France;; )
  • Triberti Paolo (Museo Civ Storia Nat, Verona, Italy;; )
  • Ohshima Issei (Kyoto Prefectural Univ, Dept Life & Environm Sci, Kyoto, Japan;; )
  • Haran Julien (INRA, Zool Forestiere UR0633, Orleans, France;; ; IRD, CIRAD, INRA, UMR CBGP,SupAgro, Montpellier, France;; )
  • Byun Bong-Kyu (Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Sukachev Inst Forest SB RAS, Krasnoyarsk, Russia;; ; Hannam Univ, Dept Biol Sci & Biotechnol, Daejeon, South Korea)
  • Li Houhun
  • Augustin Sylvie (INRA, Zool Forestiere UR0633, Orleans, France;; )
  • Roques Alain (INRA, Zool Forestiere UR0633, Orleans, France;; )
  • Lopez-Vaamonde Carlos (INRA, Zool Forestiere UR0633, Orleans, France;; )