Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1007/s00412-017-0650-9

Ключевые слова: Histone modifications, Imprinted X chromosome inactivation, Vole, Xist

Аннотация: In vole Microtus levis, cells of preimplantation embryo and extraembryonic tissues undergo imprinted X chromosome inactivation (iXCI) which is triggered by a long non-coding nuclear RNA, Xist. At early stages of iXCI, chromatin of vole inactive X chromosome is enriched with the HP1 heterochromatin-specific protein, trimethylated H3Показать полностьюK9 and H4K20 attributable to constitutive heterochromatin. In the study, using vole trophoblast stem (TS) cells as a model of iXCI, we further investigated chromatin of the inactive X chromosome of M. levis and tried to find out the role of Xist RNA. We demonstrated that chromatin of the inactive X chromosome in vole TS cells also contained the SETDB1 histone methyltransferase and KAP1 protein. In addition, we observed that Xist RNA did not contribute significantly to maintenance of X chromosome inactive state during iXCI in vole TS cells. Xist repression affected neither transcriptional silencing caused by iXCI nor maintenance of trimethylated H3K9 and H4K20 as well as HP1, KAP1, and SETDB1 on the inactive X chromosome. Moreover, the unique repertoire of chromatin modifications on the inactive X chromosome in vole TS cells could be disrupted by a chemical compound, DZNep, and then restored even in the absence of Xist RNA. However, Xist transcript was necessary for recruitment of an additional repressive histone modification, trimethylated H3K27, to the inactive X chromosome during vole TS cell differentiation. © 2017 Springer-Verlag GmbH Germany, part of Springer Nature

Журнал: Chromosoma

Номера страниц: 1-11

ISSN журнала: 00095915

Издатель: Springer Science and Business Media Deutschland GmbH

• Shevchenko A.I. (Federal Research Centre “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Prospekt Lavrentyeva, 10, Novosibirsk, Russian Federation, Institute of Сhemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation, E.N. Meshalkin National Medical Research Centre, Ministry of Health Care of Russian Federation, Novosibirsk, Russian Federation, Novosibirsk State University, Novosibirsk, Russian Federation)
• Grigor’eva E.V. (Federal Research Centre “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Prospekt Lavrentyeva, 10, Novosibirsk, Russian Federation, Institute of Сhemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation, E.N. Meshalkin National Medical Research Centre, Ministry of Health Care of Russian Federation, Novosibirsk, Russian Federation, Novosibirsk State University, Novosibirsk, Russian Federation)
• Medvedev S.P. (Federal Research Centre “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Prospekt Lavrentyeva, 10, Novosibirsk, Russian Federation, Institute of Сhemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation, E.N. Meshalkin National Medical Research Centre, Ministry of Health Care of Russian Federation, Novosibirsk, Russian Federation, Novosibirsk State University, Novosibirsk, Russian Federation)
• Zakharova I.S. (Federal Research Centre “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Prospekt Lavrentyeva, 10, Novosibirsk, Russian Federation, Institute of Сhemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation, E.N. Meshalkin National Medical Research Centre, Ministry of Health Care of Russian Federation, Novosibirsk, Russian Federation)
• Dementyeva E.V. (Federal Research Centre “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Prospekt Lavrentyeva, 10, Novosibirsk, Russian Federation, Institute of Сhemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation, E.N. Meshalkin National Medical Research Centre, Ministry of Health Care of Russian Federation, Novosibirsk, Russian Federation)
• Elisaphenko E.A. (Federal Research Centre “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Prospekt Lavrentyeva, 10, Novosibirsk, Russian Federation, Institute of Сhemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation, E.N. Meshalkin National Medical Research Centre, Ministry of Health Care of Russian Federation, Novosibirsk, Russian Federation)
• Malakhova A.A. (Federal Research Centre “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Prospekt Lavrentyeva, 10, Novosibirsk, Russian Federation, Institute of Сhemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation, E.N. Meshalkin National Medical Research Centre, Ministry of Health Care of Russian Federation, Novosibirsk, Russian Federation, Novosibirsk State University, Novosibirsk, Russian Federation)
• Pavlova S.V. (Federal Research Centre “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Prospekt Lavrentyeva, 10, Novosibirsk, Russian Federation, Institute of Сhemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation, E.N. Meshalkin National Medical Research Centre, Ministry of Health Care of Russian Federation, Novosibirsk, Russian Federation)
• Zakian S.M. (Federal Research Centre “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Prospekt Lavrentyeva, 10, Novosibirsk, Russian Federation, Institute of Сhemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation, E.N. Meshalkin National Medical Research Centre, Ministry of Health Care of Russian Federation, Novosibirsk, Russian Federation, Novosibirsk State University, Novosibirsk, Russian Federation)

• Scopus