Four steps for revealing and adjusting the 3D structure of aptamers in solution by small-angle X-ray scattering and computer simulation

Описание

Год издания: 2019

Идентификатор DOI: 10.1007/s00216-019-02045-0

Ключевые слова: Aptamer; Thrombin; Three-dimensional structure; Small-angle X-ray scattering; Molecular modeling

Аннотация: Nucleic acid (NA) aptamers bind to their targets with high affinity and selectivity. The three-dimensional (3D) structures of aptamers play a major role in these non-covalent interactions. Here, we use a four-step approach to determine a true 3D structure

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Издание

Журнал: ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Выпуск журнала: Vol. 411, Is. 25

Номера страниц: 6723-6732

ISSN журнала: 16182642

Место издания: HEIDELBERG

Издатель: SPRINGER HEIDELBERG

Авторы

  • Tomilin Felix N. (Russian Acad Sci, Kirensky Inst Phys, Fed Res Ctr KSC, Siberian Branch, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia; Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia)
  • Moryachkov Roman (Russian Acad Sci, Kirensky Inst Phys, Fed Res Ctr KSC, Siberian Branch, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia; Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, 50 Akademgorodok, Krasnoyarsk 660036, Russia)
  • Shchugoreva Irina (Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia; Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, 50 Akademgorodok, Krasnoyarsk 660036, Russia)
  • Zabluda Vladimir N. (Russian Acad Sci, Kirensky Inst Phys, Fed Res Ctr KSC, Siberian Branch, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia)
  • Peters Georgy (NRC Kurchatov Inst, 1 Acad Kurchatov Sq, Moscow 123182, Russia)
  • Platunov Mikhail (Russian Acad Sci, Kirensky Inst Phys, Fed Res Ctr KSC, Siberian Branch, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia)
  • Spiridonova Vera (Lomonosov Moscow State Univ, AN Belozersky Inst Physicochem Biol, 1-40 Leninskie Gory, Moscow 119992, Russia)
  • Melnichuk Anastasia (Lomonosov Moscow State Univ, AN Belozersky Inst Physicochem Biol, 1-40 Leninskie Gory, Moscow 119992, Russia)
  • Atrokhova Anastasia (Lomonosov Moscow State Univ, AN Belozersky Inst Physicochem Biol, 1-40 Leninskie Gory, Moscow 119992, Russia)
  • Zamay Sergey S. (Russian Acad Sci, Kirensky Inst Phys, Fed Res Ctr KSC, Siberian Branch, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia; Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, 50 Akademgorodok, Krasnoyarsk 660036, Russia)
  • Ovchinnikov Sergey G. (Russian Acad Sci, Kirensky Inst Phys, Fed Res Ctr KSC, Siberian Branch, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia; Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia)
  • Zamay Galina S. (Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, 50 Akademgorodok, Krasnoyarsk 660036, Russia; Krasnoyarsk State Med Univ, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia)
  • Sokolov Alexey (Russian Acad Sci, Kirensky Inst Phys, Fed Res Ctr KSC, Siberian Branch, 50-38 Akademgorodok, Krasnoyarsk 660036, Russia; Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia)
  • Zamay Tatiana N. (Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, 50 Akademgorodok, Krasnoyarsk 660036, Russia; Krasnoyarsk State Med Univ, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia)
  • Berezovski Maxim V. (Univ Ottawa, Dept Chem & Biomol Sci, 10 Marie Curie, Ottawa, ON K1N 6N5, Canada)
  • Kichkailo Anna S. (Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Siberian Branch, 50 Akademgorodok, Krasnoyarsk 660036, Russia)

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