Тип публикации: статья из журнала

Год издания: 2015

Идентификатор DOI: 10.1007/s11010-014-2227-8

Ключевые слова: CD4+ T cells, Differentiation, Galectin-1, Transcription factors, galectin 1, messenger RNA, retinoid related orphan receptor gamma, transcription factor GATA 3, transcription factor T bet, forkhead transcription factor, FOXP3 protein, human, RORC protein, human, T box transcription factor, T-box transcription factor TBX21, apoptosis, Article, CD4+ T lymphocyte, cellular immunity, concentration response, controlled study, down regulation, gene expression regulation, human, human cell, lymphocyte differentiation, peripheral blood mononuclear cell, regulatory T lymphocyte, T cell culture, Th2 cell, transcription initiation, transcription regulation, upregulation, cell culture, cell differentiation, dose response, drug effects, gene expression, genetics, metabolism, mononuclear cell, reverse transcription polymerase chain reaction, CD4-Positive T-Lymphocytes, Cells, Cultured, Dose-Response Relationship, Drug, Forkhead Transcription Factors, GATA3 Transcription Factor, Humans, Leukocytes, Mononuclear, Nuclear Receptor Subfamily 1, Group F, Member 3, Reverse Transcriptase Polymerase Chain Reaction, T-Box Domain Proteins

Аннотация: CD4+ T cells orchestrate the immune response by differentiating into T helper (Th) or regulatory (Treg) cell subsets that secrete distinct sets of cytokines. They also play a critical role in the pathogenesis of autoimmunity, asthma, allergy and, likely, cancer. The mechanisms involved in the regulation of CD4+ T cell homeostasis bПоказать полностьюy galectin-1 remain poorly characterized. To investigate whether galectin-1 modulates the differentiation of CD4+ T cells, the effects of galectin-1 on the mRNA expression levels of TBX21, GATA-3, FOXP3 and RORC in activated peripheral blood mononuclear cells were examined. The expression levels of GATA-3 and FOXP3 mRNA were up-regulated after treatment with 1.0 ?g/ml galectin-1 and were unchanged (for GATA-3) or slightly elevated (for FOXP3) compared with untreated cells when 2.0 ?g/ml galectin-1 was added. At the same time, at both concentrations of galectin-1, we observed reduced TBX21 and RORC mRNA expression levels. These findings support the concept that galectin-1 skews the differentiation of CD4+ T cells towards Th2 and Treg cells. © 2014, Springer Science+Business Media New York.

Журнал: Molecular and Cellular Biochemistry

Выпуск журнала: Vol. 398, Is. 1-2

Номера страниц: 245-249

• Yakushina V.D. (Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russian Federation)
• Vasil’eva O.A. (Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russian Federation)
• Ryazantseva N.V. (Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russian Federation, National Research Tomsk State U)
• Novitsky V.V. (Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russian Federation, National Research Tomsk State Univ)
• Tashireva L.A. (Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russian Federation)

• Scopus